PMID: 6403870Apr 7, 1983Paper

Formation of proinsulin by immobilized Bacillus subtilis

Nature
K MosbachL Bülow

Abstract

There has been an increasing interest in the use of immobilized cells for the production of pharmaceuticals as well as for products such as high fructose syrup or ethanol. Some of these compounds are now produced on an industrial scale whereby the cells are used in a resting or growing state or in a nonviable form as natural carriers of the enzyme(s) involved in the synthesis. The advantages of immobilized cell technology should also apply to microorganisms modified by recombinant DNA techniques to produce a variety of eukaryotic proteins such as hormones. We describe here the properties of immobilized Bacillus subtilis cells carrying plasmids encoding rat proinsulin. Cell proliferation normally coupled to DNA replication is undesirable in immobilized cell systems as "clogging' of the system occurs due to cells growing outside the beads. Therefore, different ways were investigated to inhibit cell division while allowing continued protein synthesis. We found that the addition of certain antibiotics in the growth medium, such as novobiocin which inhibits DNA replication, fulfills these requirements, allowing proinsulin synthesis and excretion to take place over a period of several days.

References

Dec 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·M GellertJ Tomizawa
Apr 7, 1972·Biochimica Et Biophysica Acta·H NilssonK Mosbach
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·K TalmadgeW Gilbert

❮ Previous
Next ❯

Citations

Aug 5, 1991·Biotechnology and Bioengineering·R H KuhnD F Ollis
Mar 1, 1994·Enzyme and Microbial Technology·V V VyasP Sharma
Jun 1, 1990·Journal of Biotechnology·P K Kumar, K Schügerl
Jan 1, 1994·Biotechnology Advances·A J McLoughlin
May 3, 1984·The New England Journal of Medicine·D C RobbinsA H Rubenstein
Jan 1, 1990·Annals of the New York Academy of Sciences·J N BarbotinD Thomas
May 2, 1994·Annals of the New York Academy of Sciences·J N Barbotin
Jan 1, 1985·Gene·K NakahamaM Kikuchi
Jul 1, 1989·Biotechnology and Bioengineering·S F Karel, C R Robertson
Jan 1, 1987·Annals of the New York Academy of Sciences·F M IníestaJ N Barbotin
Jan 1, 1984·Annals of the New York Academy of Sciences·K Mosbach
Jan 1, 1987·Annals of the New York Academy of Sciences·K BaileyG K Chotani
May 1, 1985·Journal of Bacteriology·S KovacevicJ R Miller
Mar 1, 1993·Microbiological Reviews·M Simonen, I Palva

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.