PMID: 7334525Jan 1, 1981Paper

Formation of pyrophosphate, tripolyphosphate, and phosphorylimidazole with the thioester, n, s-diacetyl-cysteamine, as the condensing agent

Journal of Molecular Evolution
A L Weber

Abstract

Reaction of 0.20M orthophosphate with 0.20M N,S-diacetylcysteamine in 0.40M imidazole at pH 7.0 or 8.0 under drying conditions at 50 degree C for 6 days yields pyrophosphate and tripolyphosphate in the presence and absence of 0.10M divalent metal ion. The efficiency of utilization of N,S-diacetylcysteamine in the formation of pyrophosphate linkages ranges from 3 - 8% under the above conditions. The thioester, N,S-diacetylcysteamine, and imidazole are required for phosphoanhydride formation. Reaction of 0.40M orthophosphate with 0.20M N,S-diacetylcysteamine in 0.40M orthophosphate with 0.20M N,S-diacetylcysteamine in 0.40M imidazole at ambient temperature for 6 days yields phosphorylimidazole in the absence or presence of 0.50M MgCl2. Phosphorylimidazole and pyrophosphate are formed in the presence of 0.05M CaCl2; pyrophosphate and tripolyphosphate are formed with 0.15M CaCl2. The efficiency of utilization of N,S-diacetylcysteamine in the formation of pyrophosphate linkages is roughly 7% at 6 days of reaction with 0.15M CaCl2. The thioester, N,S-diacetylcysteamine and imidazole are required for the formation of phosphoanhydrides. The significance of these reactions to molecular evolution is discussed.

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Citations

Jan 1, 1990·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·M Hermes-LimaA Vieyra
Jan 1, 1989·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·A L Weber
Jan 1, 1987·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·A L Weber
Jan 1, 1990·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·A L Weber, V Hsu
Jan 1, 1989·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·A L Weber
Jan 1, 1984·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·A L Weber
Jan 1, 1983·Journal of Molecular Evolution·A L Weber
Jan 1, 1987·Journal of Molecular Evolution·A L Weber
Jan 1, 1985·Journal of Molecular Evolution·A L Weber
Jan 1, 1991·Journal of Molecular Evolution·A L Weber
Dec 1, 2006·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·William J HaganMeagan Hathaway
Jan 16, 2008·Proceedings of the National Academy of Sciences of the United States of America·Matthew A Pasek
Jan 27, 2007·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·William Martin, Michael J Russell
Jun 12, 2013·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Filipa L SousaWilliam F Martin
May 11, 2011·Artificial Life·Andrew J Pratt
Jun 1, 1995·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·A L Weber
Mar 10, 2017·Astrobiology·Radosław W Piast, Rafał M Wieczorek
Jun 24, 2017·Journal of the American Chemical Society·Albert C FahrenbachJack W Szostak
Jul 14, 2017·Life·Christian Fernández-GarcíaMatthew W Powner
Sep 4, 1997·Nature·R Liu, L E Orgel
Dec 30, 2009·Chembiochem : a European Journal of Chemical Biology·William J Hagan
Jan 17, 2019·Clinical Linguistics & Phonetics·Karl VelikRobert Allen Fox
Oct 3, 2009·Journal of Speech, Language, and Hearing Research : JSLHR·Claire H DwyerHarvey R Gilbert
Sep 9, 2020·Chemical Communications : Chem Comm·Sebastian A SandenShawn E McGlynn
Jun 3, 2021·Life·Hillary H SmithSarah Stewart Johnson

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