PMID: 9895225Jan 23, 1999Paper

Formation of reactive oxygen species following bioactivation of gentamicin

Free Radical Biology & Medicine
S H Sha, J Schacht

Abstract

The present study investigated the ability of gentamicin to catalyze free radical reactions and probed the underlying mechanisms by hydroethidine imaging, oxygen consumption, and reduction of cytochrome c. In Epstein-Barr virus-transformed lymphoblastoid cells, a respiratory burst was induced by phorbol ester and detected by hydroethidine, a fluorescent indicator of superoxide radical. The addition of gentamicin increased the fluorescence two-fold while gentamicin did not produce fluorescence in the absence of phorbol ester. In membrane preparations, gentamicin did not enhance NADPH consumption ruling out a direct activation of NADPH oxidase. The formation of reactive oxygen species by gentamicin was additionally supported by experiments that showed gentamicin increased oxygen consumption two-fold in intact cells and a cell-free system. In addition, generation of superoxide was indicated by the gentamicin-stimulated reduction of cytochrome c. The stimulation by gentamicin depended upon the presence of iron (FeII/FeIII) and of arachidonic acid as an electron donor. These results support the hypothesis that an iron-gentamicin complex can increase reactive oxygen species in nonenzymatic and in biological systems. The requirement f...Continue Reading

References

Jan 1, 1979·Archives of Oto-rhino-laryngology·J Schacht
Sep 1, 1979·Hoppe-Seyler's Zeitschrift für physiologische Chemie·H C BauerG Brunner
Nov 1, 1992·American Journal of Medical Genetics·R T PrezantN Fischel-Ghodsian
Apr 15, 1992·Biochemical Pharmacology·S A CrannJ Schacht
Dec 1, 1990·Biochemical Pharmacology·M Y Huang, J Schacht
Jul 1, 1986·The American Journal of Physiology·S Grinstein, W Furuya
Jul 1, 1986·Antimicrobial Agents and Chemotherapy·D DulonJ Schacht
Oct 1, 1987·The American Journal of Physiology·P D Walker, S V Shah
May 1, 1981·The Journal of Antibiotics·H KuramochiH Umezawa
Mar 1, 1981·Hearing Research·M G Pierson, A R Møller
Mar 1, 1983·Hearing Research·G R BockP Moorjani
Sep 1, 1983·The Journal of Clinical Investigation·P A WardC Beauchamp
Dec 1, 1994·Environmental Health Perspectives·B M Babior
Jan 1, 1994·Methods in Enzymology·O T Jones, J T Hancock
Feb 1, 1994·Journal of Leukocyte Biology·W O CarterJ P Robinson
Nov 27, 1995·Biochemical Pharmacology·E M Priuska, J Schacht
Jul 17, 1998·Free Radical Biology & Medicine·B B SongJ Schacht

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Citations

Jan 23, 2008·HNO·S Pfannenstiel, M Praetorius
Nov 8, 2003·Journal of the Association for Research in Otolaryngology : JARO·P S SteygerC F Dai
Jun 29, 2005·Journal of the Association for Research in Otolaryngology : JARO·Ivana NagyDaniel Bodmer
Apr 2, 2003·Journal of Inorganic Biochemistry·Wojciech SzczepanikMalgorzata Jezowska-Bojczuk
Mar 19, 1999·Hearing Research·S H Sha, J Schacht
Jul 30, 2003·Free Radical Biology & Medicine·Perla D MaldonadoJosé Pedraza-Chaverrí
Oct 30, 2008·The Journal of Laryngology and Otology·L-F YeY-L Yuan
Jul 30, 2011·Integrative Biology : Quantitative Biosciences From Nano to Macro·Takatoshi Karasawa, Peter S Steyger
Feb 11, 2009·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Fabricia PetronilhoFelipe Dal-Pizzol
Jan 30, 2008·Toxicological Sciences : an Official Journal of the Society of Toxicology·Grant BledsoeJulie Chao
Jan 24, 2014·The Journal of Laryngology and Otology·V KahyaO Ozturan
Apr 26, 2014·Journal of Otolaryngology - Head & Neck Surgery = Le Journal D'oto-rhino-laryngologie Et De Chirurgie Cervico-faciale·Vinay T Fernandes, Vincent Y W Lin
Nov 29, 2011·International Journal of Otolaryngology·M E HuthA G Cheng
Jun 7, 2011·Hearing Research·Jing XieJochen Schacht
Sep 1, 2005·Basic & Clinical Pharmacology & Toxicology·Juan R Muñoz-CastañedaIsaac Túnez
Sep 8, 2004·Hearing Research·Ivana NagyDaniel Bodmer
Jul 18, 2006·Hearing Research·Yang ChenJochen Schacht
Apr 18, 2006·Biochemical and Biophysical Research Communications·Wei-Jia KongMin-Xin Guan
Oct 12, 2004·Otolaryngologic Clinics of North America·Avik Banerjee, Lorne S Parnes
Apr 18, 2015·Hearing Research·Ulf-Rüdiger HeinrichKai Helling
Oct 11, 2005·Drug Discovery Today·Leonard P Rybak, Craig A Whitworth
Aug 13, 2011·Free Radical Biology & Medicine·Sophie DenamurMarie-Paule Mingeot-Leclercq
Apr 1, 2012·Korean Journal of Audiology·Joung Uk KimJong Woo Chung
Jun 6, 2006·Advances in Carbohydrate Chemistry and Biochemistry·Bert Willis, Dev P Arya
Sep 9, 2009·Hearing Research·Eric C BielefeldDonald Henderson
Dec 15, 2000·Kidney International·P SinswatJ Schacht
Jun 17, 2004·International Journal of Audiology·Anna Rita FetoniDiana Troiani
Feb 5, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Kohei KawamotoYehoash Raphael
Aug 12, 2014·Laboratory Investigation; a Journal of Technical Methods and Pathology·Hyun-Soo ShinDuk-Hee Kang
Nov 9, 2017·Frontiers in Cellular Neuroscience·Chao-Hui YangSu-Hua Sha
Feb 28, 2003·Journal of Neuroscience Research·Lisa N GillespiePhillip L Marzella
Aug 30, 2018·Physiological Reviews·Jing Wang, Jean-Luc Puel

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