Formation of rigid, non-flight forewings (elytra) of a beetle requires two major cuticular proteins.

PLoS Genetics
Yasuyuki ArakaneMichael R Kanost

Abstract

Insect cuticle is composed primarily of chitin and structural proteins. To study the function of structural cuticular proteins, we focused on the proteins present in elytra (modified forewings that become highly sclerotized and pigmented covers for the hindwings) of the red flour beetle, Tribolium castaneum. We identified two highly abundant proteins, TcCPR27 (10 kDa) and TcCPR18 (20 kDa), which are also present in pronotum and ventral abdominal cuticles. Both are members of the Rebers and Riddiford family of cuticular proteins and contain RR2 motifs. Transcripts for both genes dramatically increase in abundance at the pharate adult stage and then decline quickly thereafter. Injection of specific double-stranded RNAs for each gene into penultimate or last instar larvae had no effect on larval-larval, larval-pupal, or pupal-adult molting. The elytra of the resulting adults, however, were shorter, wrinkled, warped, fenestrated, and less rigid than those from control insects. TcCPR27-deficient insects could not fold their hindwings properly and died prematurely approximately one week after eclosion, probably because of dehydration. TcCPR18-deficient insects exhibited a similar but less dramatic phenotype. Immunolocalization studie...Continue Reading

References

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Citations

Apr 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·Enric UreñaDavid Martín
Jul 3, 2015·Integrative and Comparative Biology·Robert RoerAmir Sagi
Jun 25, 2015·Insect Biochemistry and Molecular Biology·Yu-Xuan YeChuan-Xi Zhang
Aug 22, 2014·PLoS Genetics·Sujata S ChaudhariSubbaratnam Muthukrishnan
Jul 27, 2018·Journal of the Royal Society, Interface·Lakshminath KundanatiNicola M Pugno
Oct 17, 2018·International Journal of Molecular Sciences·Duan TanCheng Lu
Mar 5, 2015·The Journal of Biological Chemistry·Yanina-Yasmin PeschMatthias Behr
Oct 30, 2015·Insect Science·Everett BoothSarah Pinatti
Jun 13, 2018·Pesticide Biochemistry and Physiology·Gao XiongFangyin Dai
Nov 7, 2019·Pesticide Biochemistry and Physiology·Xiaojian LiuKun Yan Zhu
Apr 6, 2021·Critical Reviews in Biotechnology·Kai SunQingguo Huang

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Datasets Mentioned

BETA
HQ634478
HQ634479

Methods Mentioned

BETA
PCR

Software Mentioned

MASCOT
Flex analysis
Bio Tools
ClustalW

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