Formation of RNA Granule-Derived Capsid Assembly Intermediates Appears To Be Conserved between Human Immunodeficiency Virus Type 1 and the Nonprimate Lentivirus Feline Immunodeficiency Virus

Journal of Virology
Jonathan C ReedJaisri R Lingappa

Abstract

During immature capsid assembly in cells, human immunodeficiency virus type 1 (HIV-1) Gag co-opts a host RNA granule, forming a pathway of intracellular assembly intermediates containing host components, including two cellular facilitators of assembly, ABCE1 and DDX6. A similar assembly pathway has been observed for other primate lentiviruses. Here we asked whether feline immunodeficiency virus (FIV), a nonprimate lentivirus, also forms RNA granule-derived capsid assembly intermediates. First, we showed that the released FIV immature capsid and a large FIV Gag-containing intracellular complex are unstable during analysis, unlike for HIV-1. We identified harvest conditions, including in situ cross-linking, that overcame this problem, revealing a series of FIV Gag-containing complexes corresponding in size to HIV-1 assembly intermediates. Previously, we showed that assembly-defective HIV-1 Gag mutants are arrested at specific assembly intermediates; here we identified four assembly-defective FIV Gag mutants, including three not previously studied, and demonstrated that they appear to be arrested at the same intermediate as the cognate HIV-1 mutants. Further evidence that these FIV Gag-containing complexes correspond to assembly i...Continue Reading

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Citations

Nov 5, 2019·Frontiers in Cellular and Infection Microbiology·Aracelly Gaete-ArgelFernando Valiente-Echeverría
Feb 5, 2021·Journal of Molecular Biology·Yisong DengJames R Williamson
Mar 7, 2021·Viruses·Barkha RamnaniKrishnamurthy Malathi
Jul 27, 2021·Frontiers in Cell and Developmental Biology·Lucas A TavaresLuis L P daSilva

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