Formation of "solvent-free" black lipid bilayer membranes from glyceryl monooleate dispersed in squalene

Biophysical Journal
S H White

Abstract

A simple technique for forming "black" lipid bilayer membranes containing negligible amounts of alkyl solvent is described. The membranes are formed by the method of Mueller et al (Circulation. 1962. 26:1167.) from glyceryl monooleate (GMO) dispersed in squalene. The squalene forms an annulus to satisfy the boundary conditions of the planar bilayer but does not appear to dissolve noticeably in the bilayer itself. The specific geometric capacitance (Cg) of the membranes at 20 degrees C formed by this technique is 0.7771 +/- 0.0048 muF/cm2. Theoretical estimates of Cg for solvent-free bilayers range from 0.75 to 0.81 muF/cm2. Alkane-free GMO bilayers formed from n-octadecane by the solvent freeze-out method of White (Biochim. Biophys. Acta. 1974. 356:8) have values of Cg = 0.7903 +/- 0.0013 muF/cm2 at 20.5 degrees C. The agreement between the various values of Cg strongly suggests that the bilayers are free of squalene. DC potentials applied to the bilayers have no detectable effect on the value of Cg, as expected for solvent-free films. The ability to form bilayers essentially free of the solvent used in the forming solution makes it possible to determine the area per molecule of the surface active lipid in the bilayer. The area...Continue Reading

Citations

Aug 1, 1989·The Journal of Membrane Biology·M S Perin, R C MacDonald
Jan 1, 1985·The Journal of Membrane Biology·J P Dilger, R Benz
Jul 29, 2009·Analytical and Bioanalytical Chemistry·Claus Hélix Nielsen
Jul 20, 2012·The Journal of Membrane Biology·Andraž PolakPeter Kramar
Dec 7, 2006·Bulletin of Mathematical Biology·Liviu MovileanuAurel I Popescu
Oct 1, 1983·Biophysical Journal·L J Bruner, J E Hall
Apr 30, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Monique Roerdink LanderClaus Hélix-Nielsen
Oct 8, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Linda C M GrossMark I Wallace
Jan 4, 2008·Proceedings of the National Academy of Sciences of the United States of America·Marcus D Collins, Sarah L Keller
Mar 25, 2009·Proceedings of the National Academy of Sciences of the United States of America·Prasad V Ganesan, Steven G Boxer
Oct 27, 2010·Proceedings of the National Academy of Sciences of the United States of America·Christopher W HarlandRaghuveer Parthasarathy
Jun 27, 2012·The Journal of General Physiology·Keith M CallenbergMichael Grabe
Nov 27, 2009·Journal of the Royal Society, Interface·Jens A LundbaekOlaf S Andersen
Aug 31, 2013·The European Physical Journal. E, Soft Matter·Shashi Thutupalli, Stephan Herminghaus
Nov 7, 2013·PloS One·Martin Loynaz PrietoMerritt C Maduke
Feb 3, 2016·The European Physical Journal. E, Soft Matter·Cyrille ClaudetGladys Massiera
Aug 18, 2009·Chemistry and Physics of Lipids·Natalie Bordag, Sandro Keller
Jun 29, 2013·Biochimica Et Biophysica Acta·Alexandra I SorochkinaYuri N Antonenko
Mar 4, 2014·Biochimica Et Biophysica Acta·Agustín MangiarottiNatalia Wilke
Oct 29, 2000·Biophysical Journal·C Nielsen, O S Andersen
Apr 17, 1998·Biophysical Journal·C NielsenO S Andersen
Jan 14, 2003·Biophysical Journal·Anatoly ChernyshevSamuel Cukierman
Oct 23, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Peter J MiliantaSunghee Lee
Dec 17, 2016·Scientific Reports·Dae-Woong JeongMyung Chul Choi
Jan 4, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Michael MartinJ R Fried
Jun 1, 1995·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·A Pohorille, M A Wilson
Aug 7, 1985·Journal of Theoretical Biology·A W Muller
Jan 12, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Maria LopezSunghee Lee
Apr 24, 2002·Physical Review. E, Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics·R HirnT M Bayerl
Nov 22, 2013·Nature·Sohini MukherjeeLora V Hooper
Nov 26, 1981·Nature·D Busath, G Szabo
Mar 25, 2006·The Journal of Biological Chemistry·Alexander A SobkoWilliam A Cramer
Jan 7, 2020·Physical Chemistry Chemical Physics : PCCP·Hatsuho UsudaKazuya Saito
Jan 17, 2013·Chemical Communications : Chem Comm·Shashi ThutupalliRalf Seemann

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.