Formation of three-dimensional hepatic tissue by the bottom-up method using spheroids

Journal of Bioscience and Bioengineering
Tatsuya OkudairaToshihisa Kajiwara

Abstract

Liver regenerative medicine has attracted attention as a possible alternative to organ transplantation. To address the challenge of liver regenerative medicine, the development of a construction method has been proposed for liver tissue in vitro with a high cell density and high functionality for transplantation into patients with severe liver failure. In this study, we fabricated highly functional three-dimensional hepatic tissue by a bottom-up method using spheroids. The hepatic tissue was formed by stacking hepatocyte spheroids covered with human umbilical vein endothelial cells (HUVECs). Hepatic tissue constructs were evaluated for cell survival, liver-specific functions, and histologically. As a result, we identified improvements in liver-specific functions (ammonia removal and albumin secretion) and cell survival. In addition, HUVECs were regularly distributed at every 100 μm within the tissue, and live cells were present within the whole tissue construct throughout the culture period. In summary, we successfully fabricated highly functional hepatic tissue by the bottom-up method using HUVEC-covered hepatocyte spheroids.

References

May 12, 2004·Biotechnology and Bioengineering·Rachel GlicklisSmadar Cohen
Mar 29, 2007·Proceedings of the National Academy of Sciences of the United States of America·Elliot E Hui, Sangeeta N Bhatia
Jul 22, 2008·Journal of Bioscience and Bioengineering·Akira ItoMasamichi Kamihira
Nov 18, 2008·Biotechnology and Bioengineering·Adam P RagoJeffrey R Morgan
Jan 30, 2009·Biomaterials·Vladimir MironovRoger R Markwald
Mar 27, 2009·Tissue Engineering. Part a·Masakazu InamoriToshihisa Kajiwara
Sep 2, 2009·Proceedings of the National Academy of Sciences of the United States of America·Srivatsan KidambiYaakov Nahmias
Sep 9, 2010·Biotechnology and Bioengineering·Jeanette BierwolfJoerg M Pollok
Jun 22, 2012·Journal of Bioscience and Bioengineering·Nana ShirakigawaTakayuki Takei
Feb 20, 2013·Scientific Reports·Katsuhisa SakaguchiTeruo Okano
Jun 19, 2013·Journal of Bioscience and Bioengineering·Nana ShirakigawaHiroyuki Ijima
Jul 3, 2013·Journal of Bioscience and Bioengineering·Shintaro Nakamura, Hiroyuki Ijima

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Citations

Oct 19, 2016·Trends in Biotechnology·Matthias W Laschke, Michael D Menger
Jan 31, 2017·Journal of Bioscience and Bioengineering·Tatsuya OkudairaToshihisa Kajiwara
Dec 6, 2017·Advanced Healthcare Materials·Pradip Shahi ThakuriHossein Tavana
Jan 13, 2018·Scientific Reports·E BaumanC C Barrias
Apr 21, 2018·Journal of Materials Science. Materials in Medicine·Binbin ZhangYi Gao
Nov 25, 2020·Tissue Engineering. Part B, Reviews·Elana M MeijerCaroline Cheng
Jan 1, 2020·Progress in Biomedical Engineering·Guang YangJohn P Fisher
Aug 6, 2021·Advanced Science·Alessandra DellaquilaTeresa Simon-Yarza

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