Formation of transient non-protein calcium pores by lysophospholipids in S49 Lymphoma cells

The Journal of Membrane Biology
Heather A Wilson-AshworthJohn D Bell

Abstract

Palmitoyl-lysophosphatidylcholine promotes a transient calcium influx in lymphoma cells. Previously, it was observed that this influx was accompanied by a temporary increase in propidium iodide permeability that appeared linked to calcium entry. Those studies demonstrated that cobalt or nickel could block the response to lysophosphatidylcholine and raised the question of whether the calcium conductance involved specific channels. This communication describes a series of experiments to address that issue. The time dependence and structural specificity of the responses to lysophosphatidylcholine reinforced the hypothesis of a specific channel or transporter. Nevertheless, observations using patch clamp or calcium channel blockers suggested that this "channel" does not involve proteins. Alternative protein-mediated mechanisms such as indirect involvement of the sodium-calcium exchanger and the sodium-potassium ATPase were also excluded. Experiments with extracellular and intracellular calcium chelators suggested a common route of entry for calcium and propidium iodide. More directly, the ability of lysophosphatidylcholine to produce cobalt-sensitive permeability to propidium iodide was reproduced in protein-free artificial membran...Continue Reading

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Citations

May 19, 2007·The Journal of Membrane Biology·Jin-Shan HuGuo-Jiang Zhang
Aug 18, 2010·Proceedings of the National Academy of Sciences of the United States of America·James S FriedmanAnand Swaroop
Mar 23, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·David A AnderssonStuart Bevan
Jul 15, 2006·Endothelium : Journal of Endothelial Cell Research·LiChun ZhouHong Yang
Nov 22, 2012·Cellular Microbiology·Jacomine Krijnse LockerBritta Brügger
May 4, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·S Courtney FraschDonna L Bratton
Nov 30, 2012·Biochemistry·Carl E CreutzJames M Eaton
Apr 4, 2009·Toxicon : Official Journal of the International Society on Toxinology·Erik TedescoCesare Montecucco

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