PMID: 19928896DOI: 10.1021/ja901538nNov 26, 2009Paper
Formation of well-oriented microtubules with preferential polarity in a confined space under a temperature gradient
Journal of the American Chemical Society
Akira KakugoJian Ping Gong
Abstract
Tubulin polymerization in a confined space under a temperature gradient produced well-oriented microtubule assemblies with preferential polarity. We analyzed the structure and polarity of these assemblies at various levels of resolution by performing polarized light microscopy (millimeter order), fluorescence microscopy (micrometer order), and transmission electron microscopy (nanometer order).
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