Formation, regeneration, and transfection of Lactobacillus plantarum protoplasts.

Applied and Environmental Microbiology
W M CosbyW J Dobrogosz

Abstract

Protoplasts of L. plantarum were produced by mutanolysin-lysozyme digestion at 50 degrees C and regenerated at a frequency of 1.6 to 3.8%. The addition of Tween 80 to the growth medium increased the length of time required for protoplast formation. When transfected with bacteriophage B2 DNA, transfection efficiencies ranged from 25 to 230 PFU/microgram of DNA and from 2.2 X 10(-5) to 4.7 X 10(-4) PFU per recovered protoplast. Total transfectant yield was 3.7 X 10(2) to 3.4 X 10(3) per treatment. Transformations with plasmid DNA were unsuccessful.

References

Jan 1, 1987·Applied and Environmental Microbiology·P Engel
Sep 1, 1986·Applied and Environmental Microbiology·A W ShragoW J Dobrogosz
Oct 1, 1987·Applied and Environmental Microbiology·J L CasoJ E Suárez
Jan 1, 1987·Plasmid·L MorelliV Sgaramella
Nov 1, 1985·Applied and Environmental Microbiology·C A West, P J Warner
Oct 1, 1984·Applied and Environmental Microbiology·Y L Lin, H P Blaschek
Nov 1, 1984·Applied and Environmental Microbiology·L J Lee-Wickner, B M Chassy

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Citations

Jan 1, 1992·World Journal of Microbiology & Biotechnology·O RilkeH P Kleber
Mar 3, 2010·Applied Microbiology and Biotechnology·Oksana V BerezinaVladimir V Zverlov
Nov 24, 2004·FEMS Microbiology Letters·M Teresa AlegreJuan M Mesas
May 1, 1996·Microbiology·Françoise BerthierFrançoise Morel-Deville

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