Formation, Structure, and Mechanical Performance of Silk Nanofibrils Produced by Heat-Induced Self-Assembly.

Macromolecular Rapid Communications
Yuelong XiaoShengjie Ling

Abstract

The heat-induced self-assembly of silk fibroin (SF) is studied by combing fluorescence assessment, infrared nanospectroscopy, wide-angle X-ray scattering, and Derjaguin-Muller-Toporov coupled with atomic force microscopy. Several fundamental issues regarding the formation, structure, and mechanical performance of silk nanofibrils (SNFs) under heat-induced self-assembly are discussed. Accordingly, SF in aqueous solution is rod-like in shape and not micellar. The formation of SNFs occurs through nucleation-dependent aggregation, but the assembly period is variable and irregular. SF shows inherent fractal growth, and this trend is critical for the short-term assembly. The long-term assembly of SF, however, mainly involves an elongation growth process. SNFs produced by different methods, such as ethanol treatment and heat incubation, have similar secondary structure and mechanical properties. These investigations improve the in-depth understanding of fundamental issues related to self-assembly of SNFs, and thus provide inspiration and guidance in designing of silk nanomaterials.

References

May 26, 1999·International Journal of Biological Macromolecules·Y TakahashiK Yuzuriha
Jun 28, 2000·Nucleic Acids Research·C Z ZhouZ G Li
Dec 12, 2001·European Journal of Biochemistry·G LiT Yu
Aug 29, 2003·Nature·Hyoung-Joon Jin, David L Kaplan
Apr 17, 2007·Proteins·Xin ChenFritz Vollrath
Jul 17, 2008·Macromolecular Bioscience·Akira MatsumotoDavid L Kaplan
May 22, 2009·Proceedings of the National Academy of Sciences of the United States of America·Zhi LinDaiwen Yang
Oct 30, 2009·Chemical Communications : Chem Comm·Chengjie FuVollrath Fritz
Dec 22, 2009·Chemical Communications : Chem Comm·Zuguang GongZhengzhong Shao
Apr 13, 2010·Nature Nanotechnology·Jozef AdamcikRaffaele Mezzenga
Jul 28, 2011·Biomacromolecules·Shengjie LingXin Chen
Aug 2, 2011·Nature Nanotechnology·Tuomas P J Knowles, Markus J Buehler
Feb 14, 2012·ACS Nano·Cameron P BrownFederico Rosei
Mar 15, 2013·Journal of the Royal Society, Interface·Steven W Cranford
Jul 25, 2014·Biomacromolecules·Shumeng BaiHesun Zhu
Apr 15, 2016·Nano Letters·Shengjie LingMarkus J Buehler
Aug 9, 2016·Scientific Reports·F S RuggeriG Dietler
Dec 14, 2016·Chemical Reviews·Alexandre Dazzi, Craig B Prater
Apr 25, 2017·Science Advances·Shengjie LingMarkus J Buehler
Feb 16, 2018·Angewandte Chemie·Jozef Adamcik, Raffaele Mezzenga
Oct 1, 2018·Progress in Polymer Science·Shengjie LingDavid L Kaplan
Jun 28, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Feng ZhangXueguang Zhang
Dec 27, 2018·Advanced Functional Materials·Yu WangShengjie Ling
Jul 8, 2019·ACS Biomaterials Science & Engineering·Jiajia ZhongShengjie Ling
Apr 1, 2018·Nature Reviews. Materials·Shengjie LingMarkus J Buehler

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