Formin Proteins FHOD1 and INF2 in Triple-Negative Breast Cancer: Association With Basal Markers and Functional Activities

Breast Cancer : Basic and Clinical Research
Vanina D HeuserMaria Gardberg

Abstract

Basal-like breast cancer is an aggressive form of breast cancer with limited treatment options. The subgroup can be identified immunohistochemically, by lack of hormone receptor expression combined with expression of basal markers such as CK5/6 and/or epidermal growth factor receptor (EGFR). In vitro, several regulators of the actin cytoskeleton are essential for efficient invasion of basal-like breast cancer cell lines. Whether these proteins are expressed in vivo determines the applicability of these findings in clinical settings. The actin-regulating formin protein FHOD1 participates in invasion of the triple-negative breast cancer cell line MDA-MB-231. Here, we measure the expression of FHOD1 protein in clinical triple-negative breast cancers by using immunohistochemistry and further characterize the expression of another formin protein, INF2. We report that basal-like breast cancers frequently overexpress formin proteins FHOD1 and INF2. In cell studies using basal-like breast cancer cell lines, we show that knockdown of FHOD1 or INF2 interferes with very similar processes: maintenance of cell shape, migration, invasion, and proliferation. Inhibition of EGFR, PI3K, or mitogen-activated protein kinase activity does not alter...Continue Reading

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Citations

May 27, 2020·Cellular and Molecular Life Sciences : CMLS·Leticia Labat-de-Hoz, Miguel A Alonso
Jul 31, 2020·BMC Cancer·Vanina D HeuserMaria Gardberg
Jan 16, 2021·Cancer Management and Research·Marta Hałas-WiśniewskaAlina Grzanka
Jun 12, 2021·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Naziha MansuriLaura Lehtinen
Oct 8, 2021·Nature Cell Biology·Paulina Moreno-LaysecaJohanna Ivaska
Oct 27, 2021·Molecular Biology Reports·Yiting ZhaoXiaofeng Jin

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Datasets Mentioned

BETA
HPA024468

Methods Mentioned

BETA
GTPases
surgical
biopsy
transfection
xenograft

Software Mentioned

ImageJ
SPSS
IncuCyte

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