PMID: 7019262Jun 1, 1981Paper

Forms of vitamin B12 in radioisotope dilution assays

Journal of Clinical Pathology
J A Begley, C A Hall

Abstract

Since the presence of analogues of vitamin B12 (B12, cobalamin, Cbl) has been postulated as the basis for the high values obtained by some radioisotope dilution assays (RIDA) of serum Cbl we examined serum for analogues. None could be demonstrated in the extracts of serum prepared for RIDA as sought by both direct and indirect techniques. The natural forms of serum Cbl were converted to cyanocobalamin (CN Cbl) by this process of extraction which included cyanide (CN). The correctly performed RIDA for Cbl based on R binder gave higher values than a RIDA based on intrinsic factor or than by bioassay. By exclusion, the difference appeared to be due to unidentified factors rather than the presence of analogues.

References

Apr 16, 1979·Clinica Chimica Acta; International Journal of Clinical Chemistry·V Lee-OwnP J Carr
Oct 1, 1978·Analytical Biochemistry·W A Fenton, L E Rosenberg
Nov 1, 1977·Annals of Internal Medicine·L J BrandtA Wagle
Apr 1, 1969·Clinica Chimica Acta; International Journal of Clinical Chemistry·S FriednerK Levin
Mar 1, 1980·Journal of Clinical Pathology·D L MollinS M Lewis

❮ Previous
Next ❯

Citations

Apr 16, 2013·PloS One·Tore Forsingdal HardleiEbba Nexo
Oct 27, 2007·Clinical Biochemistry·Tore Forsingdal HardleiEbba Nexo
Jan 1, 1985·Critical Reviews in Oncology/hematology·Y KanoF Takaku
Sep 1, 1995·Baillière's Clinical Haematology·J H Matthews
May 1, 1982·British Journal of Haematology·I Chanarin, M Muir
Mar 17, 2017·The Journal of Physical Chemistry. B·Tomasz PieńkoJoanna Trylska

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.