Formulated glyphosate activates the DNA-response checkpoint of the cell cycle leading to the prevention of G2/M transition

Toxicological Sciences : an Official Journal of the Society of Toxicology
Julie MarcOdile Mulner-Lorillon

Abstract

A glyphosate containing pesticide impedes at 10 mM glyphosate the G2/M transition as judged from analysis of the first cell cycle of sea urchin development. We show that formulated glyphosate prevented dephosphorylation of Tyr 15 of the cell cycle regulator CDK1/cyclin B in vivo, the end point target of the G2/M cell cycle checkpoint. Formulated glyphosate had no direct effect on the dual specific cdc25 phosphatase activity responsible for Tyr 15 dephosphorylation. At a concentration that efficiently impeded the cell cycle, formulated glyphosate inhibited the synthesis of DNA occurring in S phase of the cell cycle. The extent of the inhibition of DNA synthesis by formulated glyphosate was correlated with the effect on the cell cycle. We conclude that formulated glyphosate's effect on the cell cycle is exerted at the level of the DNA-response checkpoint of S phase. The resulting inhibition of CDK1/cyclin B Tyr 15 dephosphorylation leads to prevention of the G2/M transition and cell cycle progression.

Citations

May 9, 2007·Archives of Environmental Contamination and Toxicology·N BenachourG E Séralini
Oct 31, 2009·Apoptosis : an International Journal on Programmed Cell Death·Maria Agnello, Maria Carmela Roccheri
Dec 29, 2011·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Amy Lavin WilliamsJohn M DeSesso
May 11, 2012·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·R BelléO Mulner-Lorillon
Aug 13, 2009·Journal of Toxicology and Environmental Health. Part a·C BolognesiE J P Marshall
Mar 7, 2012·Bulletin of Environmental Contamination and Toxicology·Sazada SiddiquiShoukat Saeed Khan
Dec 23, 2015·Journal of Agricultural and Food Chemistry·Hui LiDeb P Jaisi
May 9, 2016·Pesticide Biochemistry and Physiology·Laura G SchaumburgMarta D Mudry
Apr 24, 2007·Chemico-biological Interactions·Annabel Simon-LevertAnne-Marie Genevière
Aug 19, 2015·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·R MesnageG E Séralini
Apr 5, 2016·Journal of Biochemical and Molecular Toxicology·Claudia N MartiniMaría Del C Vila
May 2, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Claudia N MartiniMaría del C Vila
Apr 7, 2006·Journal of Cellular Biochemistry·Ronan Le BouffantRobert Bellé
Jul 24, 2020·Environmental Science and Pollution Research International·Jing YeChao Xu
Nov 13, 2017·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Letizia AnelloMaria Di Bernardo
Aug 11, 2010·Chemical Research in Toxicology·Alejandra PaganelliAndrés E Carrasco

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