Formulation and Characterization of Bovine Serum Albumin-Loaded Niosome

AAPS PharmSciTech
Saeid MoghassemiKobra Omidfar

Abstract

Niosomal vesicle, as a unique novel drug delivery system, is synthesized by non-ionic surfactants. Both hydrophilic and lipophilic drugs and also biomacromolecular agents, such as peptides and proteins can be encapsulated in this vesicular particle. Regarding polypeptide-based component loading, and delivery potential of the niosome, some valuable studies have been conducted in recent years. However, exploring the full potential of this approach requires fine tuned optimization and characterization approaches. Therefore, this study was conducted to achieve the following two goals. First, formulation and optimization of bovine serum albumin (BSA) load and release behavior as a function of cholesterol (CH) to sorbitan monostearate (Span 60) molar ratio. Second, investigating a cost- and time-effective polypeptide detecting method via methyl orange (MO) dye. To this aim, BSA-loaded niosomes were prepared by reversed-phase evaporation technique. The effect of CH to Sorbitan monostearate (Span 60) molar ratio on noisome entrapment efficiency (EE%) and release profile of BSA was studied using a ultraviolet (UV) spectrophotometer technique (NanoDrop 2000/2000c).Niosome with a 60% CH content showed the highest BSA EE% and release behav...Continue Reading

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Citations

Aug 10, 2016·AAPS PharmSciTech·Saeid MoghassemiKobra Omidfar
Sep 9, 2016·AAPS PharmSciTech·Muhammad Imran KhanLeena Peltonen
May 17, 2017·Artificial Cells, Nanomedicine, and Biotechnology·Seyede Elmira Yazdi RouholaminiKobra Omidfar
Oct 27, 2017·Artificial Cells, Nanomedicine, and Biotechnology·Khashayar ModaresifarHassan Niknejad
Feb 23, 2019·Expert Opinion on Drug Delivery·Ana Cláudia SantosAntónio Ribeiro
Aug 6, 2020·Nanomedicine·Sruti BheriMichael E Davis
Jan 18, 2019·Advances in Pharmacological Sciences·Mahmoud GharbaviAli Sharafi
Dec 13, 2016·AAPS PharmSciTech·Javier O Morales
Jun 30, 2019·Advanced Materials·C Wyatt ShieldsSamir Mitragotri
Dec 8, 2020·Biomacromolecules·Yoonyoung KimEun Seong Lee
Feb 6, 2021·International Journal of Pharmaceutics·Kritsanaporn TansathienWorranan Rangsimawong
Apr 8, 2021·Journal of Agricultural and Food Chemistry·Sonia MorMagdaline Franklin
Apr 11, 2021·Expert Opinion on Drug Delivery·Sérgio R S VelosoElisabete M S Castanheira
Aug 31, 2021·Journal of the Science of Food and Agriculture·Devaraju RB Surendra Nath

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Methods Mentioned

BETA
confocal microscopy
surface plasmon resonance

Software Mentioned

SPSS

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