Formulation and optimisation of novel transfersomes for sustained release of local anaesthetic

The Journal of Pharmacy and Pharmacology
Ruba BnyanMatthew Roberts

Abstract

To investigate the effect of formulation parameters on the preparation of transfersomes as sustained-release delivery systems for lidocaine and to develop and validate a new high-performance liquid chromatography (HPLC) method for analysis. Taguchi design of experiment (DOE) was used to optimise lidocaine-loaded transfersomes in terms of phospholipid, edge activator (EA) and phospholipid : EA ratio. Transfersomes were characterised for size, polydispersity index (PDI), charge and entrapment efficiency (%EE). A HPLC method for lidocaine quantification was optimised and validated using a mobile phase of 30%v/v PBS (0.01 m) : 70%v/v Acetonitrile at a flow rate of 1 ml/min, detected at 255 nm with retention time of 2.84 min. The release of lidocaine from selected samples was assessed in vitro. Transfersomes were 200 nm in size, with PDI ~ 0.3. HPLC method was valid for linearity (0.1-2 mg/ml, R2 0.9999), accuracy, intermediate precision and repeatability according to ICH guidelines. The %EE was between 44% and 56% and dependent on the formulation parameters. Taguchi DOE showed the effect of factors was in the rank order : lipid : EA ratio ˃ EA type ˃ lipid type. Optimised transfersomes sustained the release of lidocaine over 24 h. ...Continue Reading

References

Nov 24, 2001·International Journal of Pharmaceutics·M CarafaG Lucania
Sep 20, 2008·Analytical Chemistry·Sarit FriedmanRaz Jelinek
Oct 20, 2009·Trends in Pharmacological Sciences·Yogeshkumar MalamAlexander M Seifalian
Apr 2, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Wei-Wei QinXue-Yan Cui
Jul 6, 2010·International Journal of Pharmaceutics·Ghada M El ZaafaranyNahed D Mortada
Nov 3, 2010·The Journal of Pharmacy and Pharmacology·M Habib AliYvonne Perrie
Oct 1, 2010·Journal of Advanced Pharmaceutical Technology & Research·Karim Masud KaziKetousetuo Kuotsu
May 11, 2013·Oral and Maxillofacial Surgery Clinics of North America·Joseph A GiovannittiJames C Phero
Aug 21, 2013·Saudi Pharmaceutical Journal : SPJ : the Official Publication of the Saudi Pharmaceutical Society·Jadupati MalakarKalyan Kumar Sen
May 28, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Jayaganesh V NatarajanSubbu Venkatraman
Apr 1, 2005·Drug Discovery Today. Technologies·Silvia RossiCarla M Caramella
Oct 13, 2015·International Journal of Pharmaceutics·Iftikhar KhanAbdelbary Elhissi
Mar 2, 2016·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Ahmed H Al ShuwailiAlaa A Abdulrasool
Apr 7, 2016·Pharmaceutical Development and Technology·R M BarbosaM H A Santana
Aug 20, 2016·International Journal of Nanomedicine·Alam ZebJin-Ki Kim
Jan 18, 2018·Journal of Pharmaceutical Sciences·Ruba BnyanMatthew Roberts
Aug 4, 2018·Current Opinion in Anaesthesiology·Angela Lucia BaloccoAdmir Hadzic

❮ Previous
Next ❯

Citations

Apr 21, 2020·Journal of Cosmetic Dermatology·Paula Lorena BucciNoemi Zaritzky
Sep 13, 2020·Pharmaceutics·Shakthi Apsara Thejani OpathaRomchat Chutoprapat
Apr 19, 2020·Daru : Journal of Faculty of Pharmacy, Tehran University of Medical Sciences·Ruba BnyanTouraj Ehtezazi
Apr 27, 2021·Journal of Pharmaceutical Sciences·Maria ApostolouIftikhar Khan
Jun 23, 2021·Journal of Pain & Palliative Care Pharmacotherapy·Apoorva Phadke, Purnima Amin
Aug 6, 2020·Current Drug Delivery·Ritika Gupta, Amrish Kumar
Oct 19, 2020·Current Pharmaceutical Design·Drashti Patel, Bappaditya Chatterjee

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Saudi Pharmaceutical Journal : SPJ : the Official Publication of the Saudi Pharmaceutical Society
A S TripathiP M Mazumder
Saudi Pharmaceutical Journal : SPJ : the Official Publication of the Saudi Pharmaceutical Society
Lalit KumarGirish Pai K
© 2021 Meta ULC. All rights reserved