Formulation, characterization, and in vitro evaluation of quantum dots loaded in poly(lactide)-vitamin E TPGS nanoparticles for cellular and molecular imaging

Biotechnology and Bioengineering
Jie PanSi-Shen Feng

Abstract

We developed a novel system of poly(lactide acid)-d-alpha-tocopheryl polyethylene glycol 1000 succinate (PLA-TPGS) nanoparticles (NPs) for quantum dots (QDs) formulation to improve imaging effects and reduce side effects as well as to promote a sustainable imaging. The QDs-loaded PLA-TPGS NPs were prepared by a modified solvent extraction/evaporation method, which were then characterized by laser light scattering (LLS) for size and size distribution; field emission scanning electron microscopy (FESEM), atomic force microscopy (AFM) and transmission electron microscope (TEM) for surface morphology. Surface chemistry of the QDs-loaded PLA-TPGS NPs was analyzed by X-ray photoelectron microscopy (XPS) and Fourier transform infra-red spectroscopy (FTIR). Encapsulation efficiency of the QDs in the polymeric nanoparticles was measured by inductively coupled plasma optical emission spectrometry (ICP-OES). The photostability of the QDs formulated in the PLA-TPGS nanoparticles was investigated as changes in the florescence intensity versus the irradiation time. Confocal laser scanning microscopy (CLSM) was used to image the cellular uptake of the QDs-loaded NPs by MCF-7 cells. Methylthiazolyldiphenyl-tetrazolium (MTT) assay was employed ...Continue Reading

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Citations

Sep 21, 2011·Nanomedicine·Yutao LiuSi-Shen Feng
Apr 1, 2009·International Journal of Molecular Sciences·Jana DrbohlavovaJaromir Hubalek
Apr 14, 2012·Biomaterials·Zhiping ZhangSi-Shen Feng
Dec 15, 2015·International Journal of Biological Macromolecules·Rahul Pratap SinghMadaswamy S Muthu
Jan 11, 2011·Nanomedicine : Nanotechnology, Biology, and Medicine·Yunqing Wang, Lingxin Chen
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Mar 2, 2016·Nature Nanotechnology·Eunkeu OhIgor L Medintz
Jul 3, 2015·BioMed Research International·Christiana M Neophytou, Andreas I Constantinou
Feb 11, 2014·BioMed Research International·Seung-Jin HanJeffrey G Winiarz
Jun 10, 2017·International Journal of Phytoremediation·Hongxia ZhaoBaocheng Qu
Jan 24, 2017·International Journal of Biological Macromolecules·Shazia TabasumKhalid Mahmood Zia

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