Formulation, Colloidal Characterization, and In Vitro Biological Effect of BMP-2 Loaded PLGA Nanoparticles for Bone Regeneration

Pharmaceutics
Teresa Del Castillo-SantaellaJosé Manuel Peula-García

Abstract

Nanoparticles (NPs) based on the polymer poly (lactide-co-glycolide) acid (PLGA) have been widely studied in developing delivery systems for drugs and therapeutic biomolecules, due to the biocompatible and biodegradable properties of the PLGA. In this work, a synthesis method for bone morphogenetic protein (BMP-2)-loaded PLGA NPs was developed and optimized, in order to carry out and control the release of BMP-2, based on the double-emulsion (water/oil/water, W/O/W) solvent evaporation technique. The polymeric surfactant Pluronic F68 was used in the synthesis procedure, as it is known to have an effect on the reduction of the size of the NPs, the enhancement of their stability, and the protection of the encapsulated biomolecule. Spherical solid polymeric NPs were synthesized, showing a reproducible multimodal size distribution, with diameters between 100 and 500 nm. This size range appears to allow the protein to act on the cell surface and at the cytoplasm level. The effect of carrying BMP-2 co-adsorbed with bovine serum albumin on the NP surface was analyzed. The colloidal properties of these systems (morphology by SEM, hydrodynamic size, electrophoretic mobility, temporal stability, protein encapsulation, and short-term rele...Continue Reading

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Citations

Sep 26, 2020·Pharmaceutics·Joana Angélica Loureiro, Maria Carmo Pereira
Feb 23, 2020·Pharmaceutics·Pariksha Jolene KondiahViness Pillay

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Methods Mentioned

BETA
enzyme-linked immuno-sorbent assay
electrophoresis
protein assay
scanning electron microscopy
dynamic light scattering
ELISA

Software Mentioned

ImageJ

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