Förster resonance energy transfer (FRET)-based small-molecule sensors and imaging agents.

Chemical Society Reviews
Luling WuTony D James

Abstract

In this tutorial review, we will explore recent advances in the construction and application of Förster resonance energy transfer (FRET)-based small-molecule fluorescent probes. The advantages of FRET-based fluorescent probes include: a large Stokes shift, ratiometric sensing and dual/multi-analyte responsive systems. We discuss the underlying energy donor-acceptor dye combinations and emphasise their applications for the detection or imaging of cations, anions, small neutral molecules, biomacromolecules, cellular microenvionments and dual/multi-analyte responsive systems.

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Citations

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Methods Mentioned

BETA
FRET
X-ray
fluorescence cell imaging
fluorescence imaging
lipidation
flow cytometry
Fluorescence

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