Fortunellin-Induced Modulation of Phosphatase and Tensin Homolog by MicroRNA-374a Decreases Inflammation and Maintains Intestinal Barrier Function in Colitis

Frontiers in Immunology
Yongjian XiongHuishu Guo

Abstract

Activation of phosphatase and tensin homolog (PTEN) is known to induce cell apoptosis. MicroRNA-374a (miR-374a), which can suppress PTEN expression, has been found abnormally expressed in inflammatory bowel disease (IBD). Fortunellin is a citrus flavonoid that is a potential anti-inflammation agent in inflammatory diseases. The present study investigated the effects and mechanisms underlying fortunellin-induced inhibition of PTEN in IBD. Colitis was established in rats by the intracolonic administration of 2,4,6-trinitrobenzene sulfonic acid to mimic human ulcerative colitis, which is the main type of IBD. miR-374a expression was measured by quantitative real-time polymerase chain reaction, and the regulation of PTEN by miR-374a was evaluated by dual luciferase reporter assay. Western blotting was used to measure the corresponding protein expression. Fortunellin ameliorated colitis symptoms, including excessive inflammation and oxidative stress. Fortunellin decreased epithelial cell apoptosis through inhibiting PTEN expression in colitis. Fortunellin-induced downregulation of PTEN could be counteracted by miR-374a depletion. Moreover, knockdown of miR-374a in vivo partly inhibited the effects of fortunellin on rat colitis. In c...Continue Reading

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Citations

Nov 22, 2019·Frontiers in Immunology·Ruibing YangXi Sun
Feb 23, 2020·Frontiers in Pharmacology·Siyu FuTaoyou Zhou
Jan 14, 2021·Critical Reviews in Food Science and Nutrition·Yue WangChong-De Sun
May 3, 2021·Burns : Journal of the International Society for Burn Injuries·Chao QinYonghao Yu
Jan 9, 2022·Reviews in Medical Virology·Subrat Kumar SwainJayashankar Das

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Methods Mentioned

BETA
flow cytometry
Assay
IEC-6
ELISA
PCR
fluorescence microscopy
transfection

Software Mentioned

GraphPad Prism
Oligoengine
Image Lab

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis