Forward genetic screen of human transposase genomic rearrangements

BioRxiv : the Preprint Server for Biology
Anton G HenssenAlex Kentsis

Abstract

Numerous human genes encode potentially active DNA transposases or recombinases, but our understanding of their functions remains limited due to shortage of methods to profile their activities on endogenous genomic substrates. To enable functional analysis of human transposase-derived genes, we combined forward chemical genetic hypoxanthine-guanine phosphoribosyltransferase 1 (HPRT1) screening with massively parallel paired-end DNA sequencing and structural variant genome assembly and analysis. Here, we report the HPRT1 mutational spectrum induced by the human transposase PGBD5, including PGBD5-specific signal sequences (PSS) that serve as potential genomic rearrangement substrates. The discovered PSS motifs and high-throughput forward chemical genomic screening approach should prove useful for the elucidation of endogenous genome remodeling activities of PGBD5 and other domesticated human DNA transposases and recombinases.

Related Concepts

Transposase
HPRT1 gene
Transposase Activity
Genome
Genes
Genomic Stability
Genetic Screening (Procedure)
Genome Assembly Sequence
Genomic Screening
PGBD5 gene

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