Fos induction in subtypes of cerebrocortical neurons following single picrotoxin-induced seizures

Brain Research
J J HiscockJ O Willoughby

Abstract

In adult rats single seizures of varying behavioural severities were caused by slow, systemic infusion of picrotoxin, an antagonist of the C1- channel at the GABAA receptor. We used a double labelling immunohistochemical method to define the subclasses of neurons that contained Fos protein following seizures. In four cortical regions (piriform, entorhinal, motor and sensory) neuronal subclasses were defined with antibodies against the calcium-binding proteins D-28K, parvalbumin and calretinin (aspiny neurons), and neurofilament protein (spiny neurons). The remaining spiny neuron population was estimated by subtraction of defined subclasses from total neuronal numbers determined from Nissl stain. After seizures, most of the calbindin D-28K immunoreactive interneurons (> 80%) and many of the unlabelled spiny neurons (60-80%) were FOs positive. Co-localisation of Fos was found in about 30% of parvalbumin, calretinin and neurofilament protein immunoreactive neurons. Paradoxically, mild seizures were associated with induction of Fos in up to 80% of cortical cells and more severe seizures with 60%, the difference being due to different levels of Fos induction in spiny neurons. These results also demonstrate that seizures induce Fos p...Continue Reading

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Citations

Dec 18, 2001·Journal of Neuroscience Research·J J HiscockJ O Willoughby
Sep 26, 2002·Journal of Chemical Neuroanatomy·M T K KirkcaldieJ C Vickers
Mar 12, 2002·The European Journal of Neuroscience·Shuzo SakataYoshio Sakurai
Oct 31, 2002·The European Journal of Neuroscience·Estel Van der GuchtLutgarde Arckens
Feb 7, 2001·Learning & Memory·R K FilipkowskiL Kaczmarek
Jul 25, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Gwendolen E Haley, Francis W Flynn
Dec 21, 2006·Somatosensory & Motor Research·Jochen F Staiger
Jan 24, 2020·Cytoskeleton·Christopher J Bott, Bettina Winckler

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