Fos protein is required for the re-expression of angiotensin II type 1 receptors in the nucleus tractus solitarii after baroreceptor activation in the rat

Neuroscience
L L WangJ Y Chan

Abstract

We evaluated in Sprague--Dawley rats the hypothesis that Fos protein induced by baroreceptor activation in the nucleus tractus solitarii participates in transcriptional regulation of the expression of angiotensin receptor genes. Reverse transcription-polymerase chain reaction revealed that baroreceptor activation elicited by sustained hypertension resulted in a transient decrease in angiotensin II subtype 1, but not subtype 2, receptor messenger RNA, in the dorsomedial medulla, including the nucleus tractus solitarii. There was subsequently a transitory reduction in the pressor response elicited by microinjection bilaterally of angiotensin II (40 pmol) into the nucleus tractus solitarii, followed by an increase in c-fos messenger RNA and Fos immunoreactivity at the same nucleus. Both the re-expression of angiotensin II subtype 1 receptor messenger RNA and restoration of pressor response to angiotensin II after baroreceptor activation were significantly blunted by bilateral application into the nucleus tractus solitarii of an antisense oligonucleotide (50 pmol) that targets against the initiation codon of c-fos messenger RNA. Control pretreatment with the corresponding sense oligonucleotide (50 pmol), or an antisense c-fos oligo...Continue Reading

References

May 31, 1991·Biochemical and Biophysical Research Communications·N IwaiT Inagami
Feb 1, 1988·Hypertension·M J Campagnole-SantosC M Ferrario
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·D Lu, M K Raizada
Mar 3, 1995·The Journal of Biological Chemistry·M A ThompsonM E Greenberg
Jul 8, 1997·Proceedings of the National Academy of Sciences of the United States of America·T C HerzigB E Markham

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