Fosfomycin and rifampin disk diffusion tests for detection of Escherichia coli mutator strains

Journal of Clinical Microbiology
Juan-Carlos GalánJosé-Luis Martinez

Abstract

A simple method, using commercial disks to detect Escherichia coli mutator strains, is proposed. The breakpoint for detecting strains with a mutation frequency >/=5 x 10(-7) was established at >/=70 and >/=20 colonies in the inhibition zone of fosfomycin and rifampin disks, respectively, after seeding 100 microl of an overnight culture. Strains with <30 and <10 colonies in fosfomycin and rifampin inhibition zones are presumptively non-mutators.

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Citations

Nov 17, 2007·Nature Reviews. Microbiology·José L MartínezDan I Andersson
Nov 19, 2011·The Journal of Antimicrobial Chemotherapy·Drosos E KarageorgopoulosMatthew E Falagas
Jan 11, 2013·The Journal of Antimicrobial Chemotherapy·Lise CrémetStéphane Corvec
Dec 30, 2009·Applied and Environmental Microbiology·Ronnie MachielsenJohan E T Van Hylckama Vlieg
Jul 11, 2006·Journal of Clinical Microbiology·M Carmen ConejoLuis Martínez-Martínez
Jan 15, 2015·Pharmaceutical Research·Jean-Paul PirnayGuy Van den Eede
Feb 26, 2011·Journal of Medical Microbiology·Anne Jolivet-GougeonPeter Tenke
Sep 10, 2016·Science·Michael BaymRoy Kishony

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