Four days of bed rest increases intrinsic mitochondrial respiratory capacity in young healthy males

Physiological Reports
Steen LarsenCarsten Lundby

Abstract

Bed rest leads to impaired glucose tolerance. Whether this is linked to maladaptation's in skeletal muscle mitochondrial function and in particular to the level of reactive oxygen species (ROS) is at present unknown. The aim of this longitudinal study was to quantify skeletal muscle mitochondrial function (respiratory capacity and ROS production) together with glucose tolerance after 4 days of strict bed rest in healthy young male subjects (n = 14). Mitochondrial function was determined in permeabilized muscle fibers using high-resolution respirometry and fluorometry, mitochondrial content (citrate synthase [CS] activity) and antioxidant protein expression levels were assessed in parallel to this. Glucose tolerance was determined by means of oral glucose tolerance tests. Intrinsic mitochondrial respiratory capacity was augmented after the bed rest period (CI + IIP : 0.43 ± 0.12 vs. 0.55 ± 0.14 [pmol/sec/mg]/CS activity), due to a decreased CS activity (158 ± 39 vs. 129 ± 25 mU/mg dw.). No differences were observed in ROS production (per mg of tissue or when normalized to CS activity). Furthermore, the protein content for catalase was increased while superoxide dismutase and glutathione peroxidase remained unaffected. These find...Continue Reading

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Nov 3, 2016·Journal of Applied Physiology·S DandanellC Lundby

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Citations

Nov 14, 2019·The Journal of Physiology·Marlou L DirksGraham P Holloway
Jan 14, 2021·Primary Care Diabetes·Fatemeh MirzaeiFarhad Farahani

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Methods Mentioned

BETA
biopsy

Clinical Trials Mentioned

NCT02612597

Software Mentioned

GraphPad Prism
Image J

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