Four-dimensional in vivo X-ray microscopy with projection-guided gating

Scientific Reports
Rajmund MoksoHolger G Krapp

Abstract

Visualizing fast micrometer scale internal movements of small animals is a key challenge for functional anatomy, physiology and biomechanics. We combine phase contrast tomographic microscopy (down to 3.3 μm voxel size) with retrospective, projection-based gating (in the order of hundreds of microseconds) to improve the spatiotemporal resolution by an order of magnitude over previous studies. We demonstrate our method by visualizing 20 three-dimensional snapshots through the 150 Hz oscillations of the blowfly flight motor.

References

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Citations

Nov 14, 2015·Scientific Reports·William VågbergHans M Hertz
Aug 30, 2016·Physica Medica : PM : an International Journal Devoted to the Applications of Physics to Medicine and Biology : Official Journal of the Italian Association of Biomedical Physics (AIFB)·Goran LovricMarco Stampanoni
Oct 27, 2016·The Review of Scientific Instruments·Masato HoshinoNaoto Yagi
Oct 26, 2016·Current Biology : CB·Ben J Hardcastle, Holger G Krapp
Nov 29, 2017·Parasitology·James D B O'SullivanPhilip J Withers
Sep 5, 2018·Journal of Synchrotron Radiation·Francisco García-MorenoJohn Banhart
Nov 9, 2018·Journal of Synchrotron Radiation·Catalina JiménezFrancisco García-Moreno
Feb 17, 2018·Scientific Reports·Tanja Schulz-MirbachMartin Heß
Aug 23, 2019·Nature Communications·Francisco García-MorenoJohn Banhart
Dec 13, 2016·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Fritz-Olaf Lehmann, Jan Bartussek
Jun 18, 2017·Scientific Reports·Chun-Chieh WangLiang-Chi Wang
Nov 2, 2017·Journal of Synchrotron Radiation·Rajmund MoksoMarco Stampanoni
May 18, 2018·Scientific Reports·Thomas De SchryverMatthieu N Boone
Oct 4, 2017·Scientific Reports·Goran LovricMarco Stampanoni
Apr 7, 2019·Biochemical Society Transactions·Venera WeinhardtCarolyn Larabell
Mar 7, 2017·Advanced Structural and Chemical Imaging·Federica MaroneMarco Stampanoni
May 21, 2017·Scientific Reports·Alberto AstolfoAlessandro Olivo
Jun 7, 2018·Proceedings of the National Academy of Sciences of the United States of America·Jason M ColeZulfikar Najmudin
May 3, 2018·Scientific Reports·Regine GradlKaye Susannah Morgan
Oct 20, 2021·Physics in Medicine and Biology·Clément JailinSimon Rit
Sep 25, 2021·Advanced Materials·Francisco García-MorenoJohn Banhart

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Methods Mentioned

BETA
imaging techniques
X-ray
imaging technique

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