Four-dimensional multi-site photolysis of caged neurotransmitters

Frontiers in Cellular Neuroscience
Mary Ann GoVincent Ricardo Daria

Abstract

Neurons receive thousands of synaptic inputs that are distributed in space and time. The systematic study of how neurons process these inputs requires a technique to stimulate multiple yet highly targeted points of interest along the neuron's dendritic tree. Three-dimensional multi-focal patterns produced via holographic projection combined with two-photon photolysis of caged compounds can provide for highly localized release of neurotransmitters within each diffraction-limited focus, and in this way emulate simultaneous synaptic inputs to the neuron. However, this technique so far cannot achieve time-dependent stimulation patterns due to fundamental limitations of the hologram-encoding device and other factors that affect the consistency of controlled synaptic stimulation. Here, we report an advanced technique that enables the design and application of arbitrary spatio-temporal photostimulation patterns that resemble physiological synaptic inputs. By combining holographic projection with a programmable high-speed light-switching array, we have overcome temporal limitations with holographic projection, allowing us to mimic distributed activation of synaptic inputs leading to action potential generation. Our experiments uniquely...Continue Reading

Citations

Apr 22, 2015·Chemistry & Biology·Miklós KépiróAndrás Málnási-Csizmadia
Dec 17, 2014·Journal of Neuroscience Methods·Serena Bovetti, Tommaso Fellin
Jan 8, 2016·Scientific Reports·Mary Ann GoVincent Ricardo Daria
Mar 3, 2018·Organic & Biomolecular Chemistry·Dénes PálfiBalázs Rózsa
Jun 17, 2016·Nature Communications·Oscar HernandezValentina Emiliani
Oct 28, 2015·Biomedical Optics Express·Vini GautamVincent R Daria
May 17, 2017·Frontiers in Cellular Neuroscience·Julian M C ChoyVincent R Daria
Feb 28, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Tina ZhangAntonio Tricoli
Apr 21, 2017·Open Biology·Ehsan KheradpezhouhEhsan Arabzadeh
Mar 16, 2021·Frontiers in Cellular Neuroscience·Paolo Pozzi, Jonathan Mapelli
Mar 23, 2021·Biophysics Reviews·Vincent R DariaHans-A Bachor

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Methods Mentioned

BETA
fluorescence imaging

Software Mentioned

ImageJ
ViALUX
Labview

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