Four dimensional phase unwrapping of dynamic objects in digital holography

Optics Express
Gili DardikmanNatan T Shaked

Abstract

We present a new four-dimensional phase unwrapping approach for time-lapse quantitative phase microscopy, which allows reconstruction of optically thick objects that are optically thin in a certain temporal point and angular view. We thus use all four dimensions of the dynamic quantitative phase profile acquired, including the angular dimension and the temporal dimension, in addition to the x-y dimensions. We first demonstrate the capabilities of this algorithm on simulative data, enabling the quantification of the reconstruction quality relative to both the ground truth and existing unwrapping approaches. Then, we demonstrate the applicability of the proposed four-dimensional phase unwrapping algorithm by experimentally capturing a dual-angular dynamic off-axis hologram with simultaneous recording of two angular views, using multiplexing of two off-axis holograms into a single multiplexed hologram.

References

Nov 1, 1984·Molecular and Cellular Biology·B J BrewerW L Fangman
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Jun 20, 2014·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Eric BarnhillNeil Roberts
Jul 1, 2014·Optics Letters·Pinhas Girshovitz, Natan T Shaked
Mar 1, 2017·Optics Express·Gili DardikmanNatan T Shaked

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Citations

Mar 16, 2019·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·Gili Dardikman, Natan T Shaked

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