Four fluorochromes for the demonstration and microfluorometric estimation of RNA

Histochemistry
S K Curtis, R R Cowden

Abstract

Microfluorometric estimates of total RNA were made in selected test material stained with berberine sulfate, acridine orange, and Hoechst 33258. These measurements were compared with those obtained with propidium iodide, which is known to interact only with double-stranded nucleic acids. It was observed that all of the fluorochromes, including propidium iodide, yielded very similar patterns of fluorescence in the various types of material tested. In isolated thymocyte and hepatocyte nuclei stained with either propidium iodide or Hoechst 33258 at pH 2, it was evident that RNA could be estimated only indirectly by measuring the amount of fluorescence before and after extraction with RNase. It was apparent that the total fluorescence of small thymocyte nuclei was affected much less by RNase extraction than that of 2c hepatocyte nuclei. Attempts to obtain direct estimates of RNA by exposing the preparations to DNase were not successful: the fluorescence of thymocyte nuclei dropped almost to zero, and hepatocyte nuclei could no longer be assigned to distinct ploidy classes. In addition, since the highly condensed chromatin of thymocyte nuclei was stained much more prominently than the looser chromatin of hepatocyte nuclei with Hoech...Continue Reading

References

Oct 1, 1975·Experimental Cell Research·Z DarzynkiewiczM R Melamed
Jan 1, 1976·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H A CrissmanJ A Steinkamp
Jan 1, 1976·Progress in Biophysics and Molecular Biology·J Moutschen
Feb 1, 1977·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·E Johannisson, B Thorell
Mar 15, 1975·Experimental Cell Research·I Hilwig, A Gropp
Sep 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J P Calvet, T Pederson
Apr 1, 1971·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R C LeifM F Austin
Sep 1, 1974·Experimental Cell Research·U TorelliR Cadossi
Mar 1, 1980·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J K Cowell, L M Franks
Jul 1, 1980·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·O S Frankfurt
Mar 1, 1980·The Histochemical Journal·G MazziniA Riccardi
Jul 1, 1980·The Journal of Cell Biology·R E JonesT E Martin
Dec 1, 1962·The Journal of Cell Biology·H YAMAGISHI

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Citations

Oct 15, 1984·Mechanisms of Ageing and Development·P K Bhattacharya, A J Pappelis
Jan 1, 1993·Cytometry·V J BirsteinB F Goncharov

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