Four genes from Pseudomonas fluorescens that encode the biosynthesis of pyrrolnitrin.

Applied and Environmental Microbiology
P E HammerJ M Ligon

Abstract

Pyrrolnitrin is a secondary metabolite of Pseudomonas and Burkholderia sp. strains with strong antifungal activity. Production of pyrrolnitrin has been correlated with the ability of some bacteria to control plant diseases caused by fungal pathogens, including the damping-off pathogen Rhizoctonia solani. Pseudomonas fluorescens BL915 has been reported to produce pyrrolnitrin and to be an effective biocontrol agent for this pathogen. We have isolated a 32-kb genomic DNA fragment from this strain that contains genes involved in the biosynthesis of pyrrolnitrin. Marker-exchange mutagenesis of this DNA with Tn5 revealed the presence of a 6.2-kb region that contains genes required for the synthesis of pyrrolnitrin. The nucleotide sequence of the 6.2-kb region was determined and found to contain a cluster of four genes that are required for the production of pyrrolnitrin. Deletion mutations in any of the four genes resulted in a pyrrolnitrin-nonproducing phenotype. The putative coding sequences of the four individual genes were cloned by PCR and fused to the tac promoter from Escherichia coli. In each case, the appropriate tac promoter-pyrrolnitrin gene fusion was shown to complement the pyrrolnitrin-negative phenotype of the corresp...Continue Reading

References

Sep 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·D E BergJ D Rochaix
Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J LavilleD Haas
Jan 1, 1992·Annual Review of Microbiology·J R Mason, R Cammack
Oct 1, 1991·Applied and Environmental Microbiology·M N VincentE A Pierson
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Oct 1, 1988·Journal of Bacteriology·F Brunel, J Davison
May 14, 1969·Journal of Molecular Biology·H W Boyer, D Roulland-Dussoix
May 1, 1980·Journal of Bacteriology·D E BergL Crossland
Dec 1, 1983·The Journal of Antibiotics·K H van PéeF Lingens
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G DittaD R Helinski
Jul 28, 1995·Science·R D FleischmannJ M Merrick
Jun 1, 1995·Bioscience, Biotechnology, and Biochemistry·T DairiM Hasegawa
Jul 1, 1994·Molecular Plant-microbe Interactions : MPMI·T D GaffneyA M Allshouse
Dec 19, 1995·Proceedings of the National Academy of Sciences of the United States of America·A SarniguetJ E Loper
Jan 1, 1996·Annual Review of Microbiology·K H van Pée
Jan 1, 1990·Biotechnology Advances·K H Van Pée
Jun 25, 1982·Science·M N Schroth, J G Hancock

❮ Previous
Next ❯

Citations

Apr 28, 2001·Journal of Experimental Botany·J M Whipps
Mar 4, 2005·Journal of Bacteriology·Tomoyasu NishizawaDavid H Sherman
Nov 4, 2008·Angewandte Chemie·Silvana FlecksKarl-Heinz van Pée
Sep 25, 2010·Applied Microbiology and Biotechnology·Young Soo KeumJeong-Han Kim
Aug 21, 2014·Environmental Microbiology Reports·Saraswoti NeupaneNils Högberg
Jul 17, 2016·Applied and Environmental Microbiology·Massimo FerraraAntonia Gallo
Aug 18, 2016·Chembiochem : a European Journal of Chemical Biology·Daniela MilbredtKarl-Heinz van Pée
Mar 18, 2000·Biological Chemistry·K H van PéeS Zehner
Apr 15, 2005·PLoS Biology·Matthew B SullivanSallie W Chisholm
Jun 15, 2007·Environmental Microbiology·Peter R WeigeleRoger W Hendrix
Sep 19, 2007·Proteins·Eduard BittoGeorge N Phillips
May 5, 2007·Chembiochem : a European Journal of Chemical Biology·Robert Winkler, Christian Hertweck
May 29, 2010·Microbiology and Molecular Biology Reviews : MMBR·Kou-San Ju, Rebecca E Parales
Jun 30, 2016·Scientific Reports·Pascal WeigoldSebastian Behrens
Dec 15, 2015·Applied and Environmental Microbiology·Jonas WickDirk Hoffmeister
Feb 26, 2016·PloS One·Daniel Garrido-SanzMiguel Redondo-Nieto
Jul 7, 2020·Acta Crystallographica. Section D, Structural Biology·Christiane WidmannHartmut H Niemann
Aug 3, 2002·Environmental Microbiology·Suhelen EganStaffan Kjelleberg
Nov 10, 2001·Annual Review of Phytopathology·CL BenderJ Loper
Mar 18, 2006·Applied Microbiology and Biotechnology·Karl-Heinz van Pée, Eugenio P Patallo
Nov 9, 2002·Molecular Plant-microbe Interactions : MPMI·Henk-jan SchoonbeekMaarten A De Waard
Oct 22, 2009·Natural Product Reports·Harald Gross, Joyce E Loper
Apr 29, 2015·Frontiers in Plant Science·Walaa K Mousa, Manish N Raizada
Dec 30, 2014·Genome Announcements·Lucy M McCullyMark W Silby
Jun 28, 2005·Nature Biotechnology·Ian T PaulsenJoyce E Loper

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.