Four glucosyltransferases, GtfJ, GtfK, GtfL and GtfM, from Streptococcus salivarius ATCC 25975.

Microbiology
C L SimpsonN A Jacques

Abstract

The four recombinant glucosyltransferases (GTFs), GtfJ, GtfK, GtfL and GtfM, that had previously been cloned from Streptococcus salivarius ATCC 25975, were individually expressed in Escherichia coli and their glucan products and kinetic properties were analysed. GtfJ was a primer-dependent GTF which synthesized an insoluble glucan composed mainly of alpha-(1-->3)-linked glucosyl residues in the presence of dextran T-10. GtfK was primer-stimulated, and produced a linear soluble dextran without any detectable branch points both in the absence and in the presence of dextran T-10. GtfL was primer-independent and produced a mixed-linkage insoluble glucan composed of approximately equal proportions of alpha-(1-->3)- and alpha-(1-->6)-linked glucosyl residues. GtfL was inhibited by dextran T-10. GtfM was primer-independent and produced a soluble dextran with approximately 5% alpha-(1-->3)-linked glucosyl residues. GtfM was essentially unaffected by the presence of dextran T-10. The results confirmed that each enzyme represented one of the four possible combinations of primer-dependency and product solubility and that each possessed unique biosynthetic properties. The soluble dextrans formed by GtfK and GtfM, as well as the mixed-linka...Continue Reading

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Citations

Mar 10, 2006·Microbiology and Molecular Biology Reviews : MMBR·Sacha A F T van HijumIneke G H van Geel-Schutten
May 15, 2013·Microbiology and Immunology·Mayu MiyanoharaNobuhiro Hanada
Mar 19, 2014·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Yasuo YoshidaFuminobu Yoshimura
Oct 15, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Christine DelormeEric Guédon
Dec 17, 2016·The Journal of Organic Chemistry·Donald W Rogers, Andreas A Zavitsas
Oct 8, 2003·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·Howard K Kuramitsu
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May 11, 1999·FEMS Microbiology Reviews·V MonchoisP Monsan
Dec 21, 2018·Frontiers in Microbiology·Rong-Rong XuWen-Mei Wang
Oct 1, 2017·Carbohydrate Polymers·Kayoko KobayashiMasahisa Wada

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