Four immunohistochemical assays to measure the PD-L1 expression in malignant pleural mesothelioma

Oncotarget
Takuya WatanabeRyoichi Nakanishi

Abstract

Immune checkpoint inhibitors (ICIs) targeting the PD-1/PD-L1 pathway are expected to be a novel therapy for combating future increases in numbers of malignant pleural mesothelioma (MPM) patients. However, the PD-L1 expression, which is a predictor of the response to ICIs, is unclear in MPM. We studied the PD-L1 expression using four immunohistochemical assays (SP142, SP263, 28-8 and 22C3) in 32 MPM patients. The PD-L1 expression in tumor cells and immune cells was evaluated to clarify the rate of PD-L1 expression and the concordance among the four assays in MPM. The positivity rate of PD-L1 expression was 53.1% for SP142, 28.1% for SP263, 53.1% for 28-8, and 56.3% for 22C3. Nine cases were positive and 10 were negative for all assays. Discordance among the four assays was found in 13 cases. The concordance rates between SP142 and 22C3 and between 28-8 and 22C3 were the highest (84.4%). The concordance rates between SP263 and the other three assays were low (71.9% to 75.0%). The PD-L1 expression in MPM was almost equivalent for three of the assays. Given the cut-off values set in our study, these findings suggested that these assays, except for SP263, can be used for accurate PD-L1 immunostaining in MPM.

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Citations

Aug 7, 2019·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Emina TorlakovicMing-Sound Tsao
Dec 24, 2019·Frontiers in Oncology·Gerard J ChuJohn E J Rasko
Oct 17, 2020·Therapeutic Advances in Medical Oncology·Liu JinZhongwen Chen
Jul 13, 2021·Frontiers in Oncology·Stefanie HiltbrunnerFederica Grosso

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Methods Mentioned

BETA
Assay
biopsy
surgical resection

Software Mentioned

Blueprint
JMP

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