Four methods of measuring human corneal endothelial cells from specular photomicrographs

Archives of Ophthalmology
G O WaringL S Rosenblatt

Abstract

We measured central corneal endothelial cell density and area from contact specular photomicrographs of ten normal and ten abnormal corneas, comparing the precision, cost, and speed of four methods: a rectangle, planimeter, digitizer, and cell sizer. The rectangle, planimeter, and digitizer gave results that differed less than 10% from each other; therefore, the three methods can be used interchangeably for clinical purposes. There are statistically significant differences among the three techniques that may be important in basic research. The cell sizer gave a rapid, less precise estimate of mean cell area and cell density. The planimeter and digitizer measured individual endothelial cell size, and the latter entered data directly into a computer that printed both a copy of the endothelial mosaic and a histogram of cell size frequency, and computed cell density and mean cell area. We make the following recommendations: Count cells in a rectangle used for routine clinical measurement. use a cell sizer for rough estimation, as in an eyebank setting. Use a computerized digitizer to study individual endothelial cell size.

Citations

Jan 1, 1995·Documenta Ophthalmologica. Advances in Ophthalmology·M LandeszG van Rij
Apr 1, 1996·American Journal of Ophthalmology·K A WalterF Edelhauser H
Jan 1, 1993·Documenta Ophthalmologica. Advances in Ophthalmology·J V SiertsemaG van Rij
Apr 1, 1984·American Journal of Ophthalmology·L W HirstH Kues
Jul 15, 1984·American Journal of Ophthalmology·S N Markowitz, J D Morin
Oct 15, 1984·American Journal of Ophthalmology·R O SchultzK J Schultz
Jan 1, 1988·The British Journal of Ophthalmology·O Nishi, K Hanasaki
Jun 1, 1988·The British Journal of Ophthalmology·A K Bates, H Cheng
Dec 1, 1981·The British Journal of Ophthalmology·N C Price, D J Barbour
Oct 1, 1996·The British Journal of Ophthalmology·I N Nartey
Jul 1, 1993·Medical & Biological Engineering & Computing·G CorkidiE Graue
May 1, 1986·International Ophthalmology·A J JacksonR A Mufti
Aug 10, 1999·Computers in Biology and Medicine·F J Sanchez-Marin
Jul 1, 1987·The Journal of Audiovisual Media in Medicine·C O Peckar
Nov 1, 1985·Australian and New Zealand Journal of Ophthalmology·D P ReinehrR M Stasiuk
Apr 5, 2017·Cell and Tissue Banking·Nigel H Brookes
Feb 24, 2005·Cornea·Christopher WirbelauerDuy Thoai Pham
Nov 1, 1985·Australian and New Zealand Journal of Ophthalmology·R M StasiukD P Reinehr
Oct 1, 1996·The British Journal of Ophthalmology·K Ohtsuka, K Saito
May 17, 2014·Optometry and Vision Science : Official Publication of the American Academy of Optometry·Charles W McMonnies
Jul 1, 1989·Journal of Cataract and Refractive Surgery·U MenchiniR Brancato
Jul 1, 1995·Journal of Cataract and Refractive Surgery·M LandeszG Van Rij
Jan 1, 1996·Journal of Cataract and Refractive Surgery·H B DickK W Jacobi
Nov 17, 2004·Optometry and Vision Science : Official Publication of the American Academy of Optometry·Clare O'Donnell, Nathan Efron
Feb 1, 1982·Acta Ophthalmologica·H B Collin, B E Grabsch
Feb 1, 1988·Current Eye Research·G W Good, J P Schoessler
Dec 1, 1990·Acta Ophthalmologica·I LehtoK Setälä
Dec 1, 1984·Acta Ophthalmologica·M Davanger, E G Olsen
Jun 1, 1985·Current Eye Research·R W YeeH F Edelhauser
Apr 1, 1983·Acta Ophthalmologica·H I Alanko
Jan 4, 2003·Ophthalmology·H Burkhard DickJan Venter

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