Oct 20, 2009

Four p67 alleles identified in South African Theileria parva field samples

Veterinary Parasitology
Kgomotso P SibekoNicola E Collins

Abstract

Previous studies characterizing the Theileria parva p67 gene in East Africa revealed two alleles. Cattle-derived isolates associated with East Coast fever (ECF) have a 129bp deletion in the central region of the p67 gene (allele 1), compared to buffalo-derived isolates with no deletion (allele 2). In South Africa, Corridor disease outbreaks occur if there is contact between infected buffalo and susceptible cattle in the presence of vector ticks. Although ECF was introduced into South Africa in the early 20th century, it has been eradicated and it is thought that there has been no cattle to cattle transmission of T. parva since. The variable region of the p67 gene was amplified and the gene sequences analyzed to characterize South African T. parva parasites that occur in buffalo, in cattle from farms where Corridor disease outbreaks were diagnosed and in experimentally infected cattle. Four p67 alleles were identified, including alleles 1 and 2 previously detected in East African cattle and buffalo, respectively, as well as two novel alleles, one with a different 174bp deletion (allele 3), the other with a similar sequence to allele 3 but with no deletion (allele 4). Sequence variants of allele 1 were obtained from field samples...Continue Reading

Mentioned in this Paper

Syncerus caffer
Electrophoresis, Agar Gel
Eterosonycha alpina
P67 antigen, Theileria
Theileriasis
Cell Culture Techniques
Theileria parva
Bos taurus
Recombination, Genetic
Gene Deletion Abnormality

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