Fourier interpolation stochastic optical fluctuation imaging

Optics Express
Simon Christoph SteinJ Enderlein

Abstract

Stochastic Optical Fluctuation Imaging (SOFI) is a super-resolution fluorescence microscopy technique which allows to enhance the spatial resolution of an image by evaluating the temporal fluctuations of blinking fluorescent emitters. SOFI is not based on the identification and localization of single molecules such as in the widely used Photoactivation Localization Microsopy (PALM) or Stochastic Optical Reconstruction Microscopy (STORM), but computes a superresolved image via temporal cumulants from a recorded movie. A technical challenge hereby is that, when directly applying the SOFI algorithm to a movie of raw images, the pixel size of the final SOFI image is the same as that of the original images, which becomes problematic when the final SOFI resolution is much smaller than this value. In the past, sophisticated cross-correlation schemes have been used for tackling this problem. Here, we present an alternative, exact, straightforward, and simple solution using an interpolation scheme based on Fourier transforms. We exemplify the method on simulated and experimental data.

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Citations

Nov 26, 2015·Nano Letters·Anna M ChizhikAlexey I Chizhik
Mar 24, 2017·Scientific Reports·Wim Vandenberg, Peter Dedecker
Jun 1, 2017·Chemical Communications : Chem Comm·Sam DuwéPeter Dedecker
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Jun 10, 2016·Journal of Biomedical Optics·Xuanze ChenYujie Sun
Nov 10, 2016·Applied Optics·Xuehua WangHanben Niu
Sep 7, 2017·Scientific Reports·Yves PeetersPeter Dedecker
Sep 13, 2019·Optics Express·Wim VandenbergPeter Dedecker

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