PMID: 9661152Jul 14, 1998Paper

Fourier transform infrared photolysis studies of caged compounds

Methods in Enzymology
V CepusKlaus Gerwert

Abstract

Time-resolved FTIR difference spectroscopy is a powerful tool for investigating molecular reaction mechanisms of proteins. In order to detect, beyond the large background absorbance of the protein and the water, absorbance bands of protein groups that undergo reactions, difference spectra have to be performed between a ground state and an activated state of the sample. Because the absorbance changes are small, the reaction has to be started in situ, in the apparatus, and in thin protein films. The use of caged compounds offers an elegant approach to initiate protein reactions with a nanosecond UV laser flash. Here, time-resolved FTIR and FT-Raman photolysis studies of the commonly used caged compounds, caged Pi, caged ATP, caged GTP, and caged calcium are presented. The use of specific isotopic labels allows us to assign the IR bands to specific groups. Because metal ions play an important role in many biological systems, their influence on FTIR spectra of caged compounds is discussed. The results presented should provide a good basis for further FTIR studies on molecular reaction mechanisms of energy or signal transducing proteins. As an example of such investigations, the time-resolved FTIR studies on the GTPase reaction of H...Continue Reading

Citations

Aug 5, 2004·The Journal of Biological Chemistry·Partha P ChakrabartiKlaus Gerwert
Feb 16, 2010·The Journal of Biological Chemistry·Bokkyoo Jun, Sunyoung Kim
Jun 19, 2012·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Philipp PinkerneilCarsten Kötting
May 11, 2005·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Carsten Kötting, Klaus Gerwert
Jul 4, 2001·European Journal of Biochemistry·F ScheirlinckxE Goormaghtigh
Jul 5, 2001·Proceedings of the National Academy of Sciences of the United States of America·C AllinK Gerwert
Sep 6, 2012·Proceedings of the National Academy of Sciences of the United States of America·Till RudackKlaus Gerwert
Dec 22, 1999·Progress in Biophysics and Molecular Biology·J L Arrondo, F M Goñi
Dec 1, 2001·Pharmacology & Therapeutics·Y ShigeriN Yumoto
Feb 16, 2007·Journal of Molecular Biology·Partha P ChakrabartiAlfred Wittinghofer
Sep 18, 2012·The Journal of Physical Chemistry. B·Paulami MandalAndreas Barth
Nov 16, 2013·The Journal of Physical Chemistry. B·Nadejda Eremina, Andreas Barth
Aug 19, 2007·Biochimica Et Biophysica Acta·Andreas Barth
Mar 28, 2002·Biophysical Journal·Blaine C ButlerGina MacDonald
Apr 20, 2002·Biophysical Journal·Aneta KirilenkoJoanna Bandorowicz-Pikula
Oct 5, 2021·Acta Crystallographica. Section D, Structural Biology·Diana C F MonteiroArwen R Pearson

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