Fourier transform infrared spectroscopic imaging and multivariate regression for prediction of proteoglycan content of articular cartilage.

PloS One
Lassi RieppoSimo Saarakkala

Abstract

Fourier Transform Infrared (FT-IR) spectroscopic imaging has been earlier applied for the spatial estimation of the collagen and the proteoglycan (PG) contents of articular cartilage (AC). However, earlier studies have been limited to the use of univariate analysis techniques. Current analysis methods lack the needed specificity for collagen and PGs. The aim of the present study was to evaluate the suitability of partial least squares regression (PLSR) and principal component regression (PCR) methods for the analysis of the PG content of AC. Multivariate regression models were compared with earlier used univariate methods and tested with a sample material consisting of healthy and enzymatically degraded steer AC. Chondroitinase ABC enzyme was used to increase the variation in PG content levels as compared to intact AC. Digital densitometric measurements of Safranin O-stained sections provided the reference for PG content. The results showed that multivariate regression models predict PG content of AC significantly better than earlier used absorbance spectrum (i.e. the area of carbohydrate region with or without amide I normalization) or second derivative spectrum univariate parameters. Increased molecular specificity favours th...Continue Reading

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Citations

Jul 8, 2014·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Jianhua Yin, Yang Xia
Sep 23, 2014·Current Osteoporosis Reports·S GamsjaegerE P Paschalis
Dec 12, 2012·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·S GamsjaegerE P Paschalis
Apr 4, 2016·Ultrasound in Medicine & Biology·Nils MännickeKay Raum
May 20, 2014·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Nora T KhanarianHelen H Lu
Apr 6, 2017·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·E P PaschalisK Klaushofer
Jul 29, 2017·Applied Spectroscopy·Mithilesh PrakashJuha Töyräs
Mar 16, 2017·Journal of Biomedical Optics·Lassi RieppoSimo Saarakkala
May 23, 2017·Tissue Engineering and Regenerative Medicine·Fatemeh AleahmadTahereh Talaei-Khozani
Feb 13, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·William QueridoNancy Pleshko

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Methods Mentioned

BETA
imaging techniques
protein folding
PCR

Software Mentioned

PLSR

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