PMID: 9431992Feb 7, 1998Paper

Fourier-transform infrared spectroscopic studies on the coordination of the side-chain COO- groups to Ca2+ in equine lysozyme

European Journal of Biochemistry
M MizuguchiK Nitta

Abstract

Interactions between Ca2+ and the Asp side chains in the Ca2+-binding site of equine lysozyme were investigated by Fourier-transform infrared (FT-IR) spectroscopy. In the spectrum of equine lysozyme, the intensities of the bands at about 1595 cm-1 and 1578 cm-1 in the region of the COO antisymmetric stretches increased upon Ca2+ binding. In the region of the COO- symmetric stretches, the loss of intensity at about 1388 cm-1 and gains of intensities at about 1423 cm-1 and 1403 cm-1 were observed due to Ca2+ binding to equine lysozyme. The spectral changes for equine lysozyme indicate that the COO- groups of Asp85, Asp90 and Asp91 in the Ca2+-binding site coordinate to Ca2+ in the pseudo-bridging mode, where divalent metal cation is bound to one of the two oxygens in the COO- group and a water molecule is hydrogen bonded to the other oxygen. The results presented here provide further evidence for a high degree of similarity between Ca2+-binding lysozyme and alpha-lactalbumin. The effects of Ca2+ binding on the main-chain conformation of equine lysozyme were compared with those of bovine alpha-lactalbumin and hen egg-white lysozyme.

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Citations

Apr 15, 2003·Chemistry and Physics of Lipids·Neslihan Toyran, Feride Severcan
Feb 28, 2001·Progress in Biophysics and Molecular Biology·A Barth
Feb 12, 2013·International Journal of Pharmaceutics·Wei HeWei Wu
Dec 7, 2007·Biophysical Journal·Vishard Ragoonanan, Alptekin Aksan
Dec 4, 2012·Biochimica Et Biophysica Acta·Masayuki NaraMasaru Tanokura
Aug 30, 2008·Biophysical Journal·Eric M JonesColette A Sacksteder
Jan 10, 2008·Biochemical and Biophysical Research Communications·Masayuki Nara, Masaru Tanokura
May 28, 2021·The Journal of Physical Chemistry. a·Sunayana MitraSean Garrett-Roe
Jan 26, 2013·Biochemistry·Belinda Pastrana-RíosMara Colón
May 8, 2020·Biochimica Et Biophysica Acta. Biomembranes·Yelena PyatskiRichard Mendelsohn

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