PMID: 14706752Jan 7, 2004

Fourier transform infrared spectroscopy as a new tool for characterization of mollicutes

Journal of Microbiological Methods
Anne-Marie MelinB de Barbeyrac

Abstract

Fourier transform infrared (FT-IR) spectroscopy is a convenient physico-chemical technique to investigate various cell materials. Bacteria of class Mollicutes, identified by conventional methods, as Mycoplasma, Acholeplasma and Ureaplasma genera were characterized using this method. A data set of 74 independent experiments corresponding to fourteen reference strains of Mollicutes was examined by FT-IR spectroscopy to attempt a spectral characterization based on the biomolecular structures. In addition to the separation of Mollicutes within the lipidic region into five main clusters corresponding to the three phylogenetic groups tested, FT-IR spectroscopy allowed a fine discrimination between strains belonging to the same species by using selective spectral windows, particularly in the 1200-900 cm(-1) saccharide range. The results obtained by FT-IR were in good agreement with both taxonomic and phylogenetic classifications of tested strains. Thus, this technique appears to be a useful tool and an accurate mean for a rapid characterization of Mollicutes observed in humans.

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Citations

Feb 2, 2006·International Journal of Radiation Biology·Nathalie GaultJean-Louis Lefaix
Dec 29, 2015·Experimental Cell Research·Cholpajsorn JunhomKanjana Thumanu
Mar 20, 2018·Analytical and Bioanalytical Chemistry·Katia WehbeGianfelice Cinque

Related Concepts

Sugars
Mollicutes
Orthophosphate
Phylogeny
Disease Clustering
Spectroscopy, Fourier Transform Infrared
Principal Component Analysis

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