Fourier transform infrared spectroscopy as a metabolite fingerprinting tool for monitoring the phenotypic changes in complex bacterial communities capable of degrading phenol

Environmental Microbiology
Emma S WharfeRoyston Goodacre

Abstract

The coking process produces great volumes of wastewater contaminated with pollutants such as cyanides, sulfides and phenolics. Chemical and physical remediation of this wastewater removes the majority of these pollutants; however, these processes do not remove phenol and thiocyanate. The removal of these compounds has been effected during bioremediation with activated sludge containing a complex microbial community. In this investigation we acquired activated sludge from an industrial bioreactor capable of degrading phenol. The sludge was incubated in our laboratory and monitored for its ability to degrade phenol over a 48 h period. Multiple samples were taken across the time-course and analysed by Fourier transform infrared (FT-IR) spectroscopy. FT-IR was used as a whole-organism fingerprinting approach to monitor biochemical changes in the bacterial cells during the degradation of phenol. We also investigated the ability of the activated sludge to degrade phenol following extended periods (2-131 days) of storage in the absence of phenol. A reduction was observed in the ability of the microbial community to degrade phenol and this was accompanied by a detectable biochemical change in the FT-IR fingerprint related to cellular p...Continue Reading

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Citations

Oct 20, 1998·Progress in Retinal and Eye Research·L Wachtmeister
Mar 10, 2015·Applied and Environmental Microbiology·Howbeer MuhamadaliRoyston Goodacre
Jun 3, 2018·Environmental Science and Pollution Research International·Xiaobiao ZhuXiang Hu
Aug 28, 2018·Chemické Zvesti·Michał KowalskiJolanta Turek-Szytow
Oct 8, 2020·Critical Reviews in Analytical Chemistry·M Fraga-CorralJ Simal-Gandara
Jul 4, 2012·Environmental Science & Technology·T Komang Ralebitso-SeniorKirsty Jarvis

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