Fourier Transform Ion Cyclotron Resonance mass spectrometry for plant metabolite profiling and metabolite identification

Methods in Molecular Biology
J William AllwoodRoyston Goodacre

Abstract

Mass spectrometry (MS) is usually the technique of choice for metabolomic studies where the volume of sample material is too limited for applications employing nuclear magnetic resonance (NMR) spectroscopy. With the advent of ultra-high accuracy mass spectrometers such as the Orbitrap (resolution ∼ 10(5)) and the Fourier Transform Ion Cyclotron Resonance (FT-ICR) analysers (resolution potentially in excess of 10(6)) there is the opportunity to generate an accurate mass fingerprint (often referred to as a profile since the variables are considered as effectively discrete) of an infused sample extract. In such data representations mass "peaks" are detected in the raw data and the centroid mass intensity calculated. The resolving power and sensitivity of these ultra-high accuracy mass analysers is such that metabolite signals from molecules containing naturally abundant elemental isotopes (e.g. (13)C, (41)K, (15)N, (17)O, (34)S, and (37)Cl) are visible in the data. Such is the instruments precision that it allows for the calculation of highly accurate elemental compositions for the unknown signals, thus aiding greatly in the selection of potential metabolite candidates for the annotation of unknowns prior to their confirmation by ...Continue Reading

Citations

Jan 27, 2015·Annual Review of Plant Biology·Ronan Sulpice, Peter C McKeown
Sep 10, 2014·Current Opinion in Biotechnology·Tobias Fuhrer, Nicola Zamboni
Oct 5, 2013·Veterinary Pathology·V G SassevilleD J Brees
Aug 30, 2014·Proteomics·Frédérique CourantBruno Le Bizec
Oct 24, 2013·The Plant Journal : for Cell and Molecular Biology·Katharina GläserErwin Grill
Nov 30, 2013·Mass Spectrometry Reviews·Christophe JunotFrançois Bécher

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