PMID: 11607094Aug 1, 1990Paper

Fourier-transform Raman spectroscopy applied to photobiological systems

Proceedings of the National Academy of Sciences of the United States of America
J SawatzkiF Siebert

Abstract

Fluorescence and initiation of photoreactions are problems frequently encountered with resonance Raman spectroscopy of photobiological systems. These problems can be circumvented with Fourier-transform Raman spectroscopy by using the 1064-nm wavelength of a continuous wave neodymium-yttrium/aluminum-garnet laser as the probing beam. This wavelength is far from the absorption band of most pigments. Yet, the spectra of the investigated systems--bacteriorhodopsin, rhodopsin, and phycocyanin--show that these systems are still dominated by the chromophore, or that preresonant Raman scattering is still prevalent. Only for rhodopsin were contributions of the protein and the membrane discernible. The spectra of phycocyanin differ considerably from those obtained by excitation into the UV-absorption band. The results show the usefulness of this method and its wide applicability. In addition, analysis of the relative preresonant scattering cross sections may provide a detailed insight into the scattering mechanism.

References

Apr 20, 1976·Biochemistry·R H CallenderK Nakanishi
Aug 1, 1988·Photochemistry and Photobiology·S P FodorR A Mathies

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Citations

Dec 27, 1990·European Journal of Biochemistry·F SiebertH Scheer
May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·L S BrownJ K Lanyi
Jan 11, 2017·The Journal of Physical Chemistry Letters·C Elias EckertJosef Wachtveitl
Sep 1, 1992·Biochemistry·P HildebrandtB Schrader
Mar 1, 2012·Physical Chemistry Chemical Physics : PCCP·Daniel B TurnerGregory D Scholes
Sep 27, 2019·Photochemistry and Photobiology·Gaoxiang MeiKenneth J Rothschild
Jul 4, 2001·The Journal of Biological Chemistry·L S BrownJ L Spudich
Mar 24, 2020·Chemical Reviews·Victor A Lorenz-Fonfria
Apr 6, 2021·Photochemistry and Photobiology·Gaoxiang MeiKenneth J Rothschild

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