Fourteen internal transcribed spacers in the circular ribosomal DNA of Euglena gracilis

Journal of Molecular Biology
M N SchnareM W Gray

Abstract

Cytoplasmic ribosomes from Euglena gracilis contain 16 rRNA components. These include the typical 5 S, 5.8 S and 19 S rRNAs that are found in other eukaryotes as well as 13 discrete small RNAs that interact to form the equivalent of eukaryotic 25-28 S rRNA (accompanying paper). We have utilized DNA sequencing techniques to establish that genes for all of these RNAs, with the exception of 5 S rRNA, are encoded by the 11,500 base-pair circular rDNA of E. gracilis. We have determined the relative positions of the coding regions for the 19 S rRNA and the 14 components (including 5.8 S rRNA) of the large subunit rRNA, thereby establishing that the genes for each of these rRNAs are separated by internal transcribed spacers. We conclude that sequences corresponding to these spacers are removed post-transcriptionally from a high molecular weight pre-rRNA, resulting in a multiply fragmented large subunit rRNA. Internal transcribed spacers, in positions analogous to some of these additional Euglena rDNA spacers, have been found in the rDNA of other organisms and organelles. This finding supports the view that at least some internal transcribed spacers may have been present at an early stage in the evolution of rRNA genes.

References

Jan 1, 1979·Methods in Enzymology·F Bolivar, K Backman
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jan 1, 1976·Annual Review of Biochemistry·R P Perry
Sep 5, 1990·Journal of Molecular Biology·M N Schnare, M W Gray
Mar 5, 1990·Journal of Molecular Biology·I L GonzalezJ E Sylvester
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M L SoginJ H Gunderson
Apr 11, 1989·Nucleic Acids Research·K Schiebel, V Hemleben
Sep 25, 1989·Nucleic Acids Research·J Rathgeber, I Capesius
Sep 1, 1987·Molecular and Cellular Biology·C G Clark, G A Cross
Jan 29, 1985·Biochimica Et Biophysica Acta·J R Cook, R Roxby
Jan 1, 1987·Journal of Molecular Evolution·C G Clark
Jul 25, 1988·Nucleic Acids Research·T KissF Solymosy
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S Tabor, C C Richardson
May 26, 1987·Nucleic Acids Research·J C BoothroydC C Wang
Oct 25, 1985·Nucleic Acids Research·P Ravel-ChapuisG Freyssinet
Mar 11, 1986·Nucleic Acids Research·R E EllisA R Coulson
Aug 14, 1971·Journal of Molecular Biology·R D Brown, R Haselkorn
Jan 1, 1982·Annual Review of Biochemistry·H BuschY C Choi
Jan 1, 1980·Methods in Enzymology·A M Maxam, W Gilbert
Jul 11, 1980·Nucleic Acids Research·G M VeldmanR Planta
Feb 10, 1984·Nucleic Acids Research·B R Hindenach, D W Stafford
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M D BigginG F Hong
Jan 1, 1982·Journal of Molecular Evolution·T KumazakiS Osawa
Jan 1, 1980·Sub-cellular Biochemistry·A A Hadjiolov

❮ Previous
Next ❯

Citations

Oct 1, 1994·Plant Molecular Biology·J S Liu, C L Schardl
Feb 7, 1996·Biochimica Et Biophysica Acta·D S SmallmanM W Gray
Nov 1, 1992·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·O Y Kwon, H Ishikawa
Apr 11, 1992·Nucleic Acids Research·M KellerP Imbault
May 11, 1992·Nucleic Acids Research·R R GutellM W Gray
Dec 11, 1999·Nucleic Acids Research·P De RijkR De Wachter
Jan 11, 2000·Nucleic Acids Research·J WuytsR De Wachter
Nov 18, 2003·Journal of Bacteriology·Dmitry ZaporojetsCatherine L Squires
Nov 18, 2009·BMC Genomics·J Michael Charette, Michael W Gray
Nov 26, 2009·FEMS Microbiology Reviews·Ana Lilia Torres-MachorroImelda López-Villaseñor
Sep 9, 2004·The Journal of Eukaryotic Microbiology·Sophie von der HeydenThomas Cavalier-Smith
Jan 19, 2020·The Journal of Biological Chemistry·Michael W Gray, Venkat Gopalan
Oct 23, 2020·Nucleic Acids Research·Donna MatzovMoran Shalev-Benami
Sep 5, 1990·Journal of Molecular Biology·M N Schnare, M W Gray

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.