Fowl Adenovirus 4 (FAdV-4)-Based Infectious Clone for Vaccine Vector Development and Viral Gene Function Studies

Viruses
Yanlong PeiEva Nagy

Abstract

Fowl adenovirus 4 (FAdV-4) is associated with economically important poultry diseases. Recent studies of fully sequenced genomes of FAdV-4 isolates suggest potential genomic regions associated with virulence and amenable for manipulation and vector development. Direct manipulation of viral genomes is cumbersome, as opposed to that of infectious clones-viral genomes cloned into plasmid or cosmid vectors. In this work, we generated an infectious clone, pFAdV-4 ON1, containing the entire viral genome of a nonpathogenic FAdV-4 (ON1 isolate). pFAdV-4 ON1 was used for targeted deletion of open reading frames (ORFs) 16 and 17 and replacement with the enhanced green fluorescence protein (EGFP) expression cassette to generate recombinant viruses. These viruses were viable, and EGFP was expressed in infected cells. Their replication, however, was significantly reduced with respect to that of the wild-type virus. These observations suggest the potential utility of FAdV-4 as a vaccine vector and the importance of ORFs 16 and 17 for virus replication at wild-type levels. To our knowledge, this is the first report of an infectious clone based on the FAdV-4 genome, and our results demonstrate its utility for studies of virulence determinants ...Continue Reading

References

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May 3, 2001·Journal of Virology·A FrançoisP Langlois
Jan 22, 2008·Virus Genes·Juan Carlos CorredorEva Nagy
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Aug 17, 2012·The Journal of General Virology·Győző L KajánMária Benkő
Dec 18, 2012·Archives of Virology·Manu AsthanaRajesh Kumar
Jun 7, 2013·Clinical and Vaccine Immunology : CVI·Li DengEva Nagy
Aug 5, 2015·Journal of Virological Methods·Yanlong PeiÉva Nagy
Nov 28, 2015·Avian Pathology : Journal of the W.V.P.A·Pedro F Vera-HernándezAngel E Absalón
May 26, 2016·Emerging Microbes & Infections·Jianqiang YeAijian Qin
Apr 5, 2017·Methods in Molecular Biology·Juan C CorredorÉva Nagy
Dec 23, 2017·Journal of Biotechnology·Yanlong PeiÉva Nagy
Jan 1, 1989·Revue Scientifique Et Technique·A E CheemaM Afzal

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Datasets Mentioned

BETA
GU188428
HE608152
KU587519
KX538980
KU558762
KX061750
KU569295
KY436520
KU569296
KY436522

Methods Mentioned

BETA
PCR
Infection

Software Mentioned

DNA Poly ( A ) Signal Miner

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