FOXD1-dependent MICU1 expression regulates mitochondrial activity and cell differentiation

Nature Communications
Santhanam ShanmughapriyaMuniswamy Madesh

Abstract

Although many factors contribute to cellular differentiation, the role of mitochondria Ca2+ dynamics during development remains unexplored. Because mammalian embryonic epiblasts reside in a hypoxic environment, we intended to understand whether mCa2+ and its transport machineries are regulated during hypoxia. Tissues from multiple organs of developing mouse embryo evidenced a suppression of MICU1 expression with nominal changes on other MCU complex components. As surrogate models, we here utilized human embryonic stem cells (hESCs)/induced pluripotent stem cells (hiPSCs) and primary neonatal myocytes to delineate the mechanisms that control mCa2+ and bioenergetics during development. Analysis of MICU1 expression in hESCs/hiPSCs showed low abundance of MICU1 due to its direct repression by Foxd1. Experimentally, restoration of MICU1 established the periodic cCa2+ oscillations and promoted cellular differentiation and maturation. These findings establish a role of mCa2+ dynamics in regulation of cellular differentiation and reveal a molecular mechanism underlying this contribution through differential regulation of MICU1.

References

Jun 20, 2001·Nature Reviews. Molecular Cell Biology·M J BerridgeM D Bootman
Dec 14, 2002·Genesis : the Journal of Genetics and Development·Simon J ConwaySrinagesh V Koushik
Sep 12, 2003·Nature·Edward M Conway, Peter Carmeliet
Jan 23, 2004·Nature·Yuriy KirichokDavid E Clapham
Feb 10, 2004·Journal of Applied Physiology·José López-BarneoPatricia Ortega-Sáenz
Oct 11, 2005·Annual Review of Cell and Developmental Biology·Linheng Li, Ting Xie
Feb 24, 2006·Nature Reviews. Molecular Cell Biology·Jean Paul Thiery, Jonathan P Sleeman
Apr 26, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Maike SchmelterHeinrich Sauer
May 4, 2006·The Journal of Clinical Investigation·Tong Yin, Linheng Li
Jul 1, 2006·Nature·David T Scadden
Feb 20, 2007·Current Biology : CB·Matthew E Carter, Anne Brunet
Dec 22, 2007·Nature Reviews. Molecular Cell Biology·D Leanne Jones, Amy J Wagers
Dec 1, 2009·Cell·Jean Paul ThieryM Angela Nieto
Dec 17, 2009·The American Journal of Pathology·Benjamin D HumphreysJeremy S Duffield
Aug 4, 2010·The Journal of Cell Biology·Brian J HawkinsMuniswamy Madesh
Aug 10, 2010·Nature·Fabiana PerocchiVamsi K Mootha
Apr 19, 2011·Nature Chemical Biology·Bailong XiaoArdem Patapoutian
May 5, 2011·Biochimica Et Biophysica Acta·David E A Kloet, Boudewijn M T Burgering
May 24, 2011·Progress in Pediatric Cardiology·George A PorterShey-Shing Sheu
May 27, 2011·Cancer Biology & Therapy·Yuqing ZhangJi-hye Paik
Jul 20, 2011·Nature Chemical Biology·Jonathan SoboloffDonald L Gill
Nov 28, 2012·Nature Cell Biology·Karthik MallilankaramanMuniswamy Madesh
Jun 12, 2013·ELife·Dipayan ChaudhuriDavid E Clapham
Jun 14, 2013·Proceedings of the National Academy of Sciences of the United States of America·George S B WilliamsW J Lederer
Dec 18, 2013·Cell Reports·Nicholas E HoffmanMuniswamy Madesh
Jan 17, 2014·Molecular Biology of the Cell·Nicholas E HoffmanMuniswamy Madesh
Feb 1, 2014·Circulation Research·Xiulan YangCharles E Murry
Feb 6, 2014·Nature Communications·Makito KogaMiki Ebisuya

❮ Previous
Next ❯

Citations

Jun 8, 2019·Toxicological Sciences : an Official Journal of the Society of Toxicology·Joseph Y CheungPhilippe Haouzi
Aug 28, 2020·EMBO Reports·Geeta RaoPriyabrata Mukherjee
Oct 2, 2019·International Journal of Molecular Sciences·Nadezhda V TarasovaAndrey V Elchaninov
May 12, 2020·Frontiers in Physiology·Sebastian MartewiczNicola Elvassore
Apr 23, 2020·Science Signaling·Neeharika NemaniMuniswamy Madesh
Mar 26, 2021·BMC Cancer·Kyle H BondLeif Oxburgh
Dec 10, 2020·American Journal of Physiology. Cell Physiology·B Rita AlevriadouMuniswamy Madesh
Oct 27, 2021·Physiological Reviews·Joanne F Garbincius, John W Elrod

❮ Previous
Next ❯

Datasets Mentioned

BETA
GM942

Methods Mentioned

BETA
ChIP
transfection
PCR
Assay
FACS

Software Mentioned

Image J
MS ProGenesis
SigmaPlot
Graphpad Prism
FlowJo
Mascot
LC
Proteome Discoverer

Related Concepts

Related Feeds

Cardiovascular Homeostasis

Cardiovascular regulation and homeostasis is important in maintaining health and dysfunction may lead to cardiovascular diseases. Nitric oxide and vascular endothelium are one of many molecules involved in the homeostatic mechanism. Here is the latest research on cardiovascular homeostasis.