FoxG1 facilitates proliferation and inhibits differentiation by downregulating FoxO/Smad signaling in glioblastoma

Biochemical and Biophysical Research Communications
Lei WangQianxue Chen

Abstract

To investigate the effects and underlying molecular mechanisms of FoxG1 expression on glioblastoma multiforme (GBM) models. Expression levels of FoxG1 and other cancer-related biomarkers were evaluated by qRT-PCR, immunoblotting and immunohistochemistry. Crystal violet staining and MTT assay and were applied in this study to verify cell proliferation ability and viability of GBM cell models with/without drug treatment. Immunohistochemical and qRT-PCR assays showed that endogenous FoxG1 expression levels were positively correlated to the GBM disease progression. Overexpression of FoxG1 protein resulted in increased cell viability, G2/M cell cycle arrest, as well as the downregulation of p21 and cyclin B1. In addition, western blot assays reported that enforced expression of FoxG1 suppressed GAPF and facilitated the expression of Sox2 and Sox5. Meanwhile the downstream targets of FoxG1, such as FoxO1 and pSmad1/5/8 were activated. Overexpression of FoxG1 under TMZ treatment restored the cell viability as well as the expression levels of Sox2 and Sox5, yet downregulated expression levels of p21 and cyclin B1. The downstream FoxG1-induced FoxO/Smad signaling was re-inhibited under TMZ treatments. Our findings suggest that FoxG1 fun...Continue Reading

Citations

Mar 12, 2020·Frontiers in Cellular Neuroscience·Pei-Shan HouCarina Hanashima
Dec 12, 2019·Frontiers in Pediatrics·Nuwan C Hettige, Carl Ernst
Jan 11, 2020·Frontiers in Oncology·Yu-Qing LiuRui-Chao Chai
Mar 15, 2020·Biochimica Et Biophysica Acta. Molecular Cell Research·Raúl VivarGuillermo Diaz-Araya
May 1, 2021·Journal of Personalized Medicine·Valentina BravatàGiorgio Russo
Oct 19, 2021·Experimental and Therapeutic Medicine·Yuan Yuan ChengFang Min Xie

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