FOXG1 mediates the radiosensitivity of glioma cells through regulation of autophagy.

International Journal of Radiation Biology
Ning XiaoMin Hong

Abstract

Upregulation of Forkhead box G1 (FOXG1) has recently been observed in many cancers, while its effect on radiosensitivity in glioma is still unclear. In this study, we hypothesized that FOXG1 be a major player in radioresistance of glioma as well as the underlying mechanism. Immunohistochemistry (IHC) was conducted to assess FOXG1 expression in glioma tissues and glioma-adjacent tissues. Western Blot was implemented to detect the expression of autophagy-related proteins. CCK-8, colony formation and flow cytometry assays were implemented to assess cell viability, proliferation and apoptosis, respectively. Transmission electron microscope (TEM) was used to observe autophagic vesicles. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assay was applied to detect the expression of FOXG1. The present study demonstrated that FOXG1 was highly expressed in glioma tissues. FOXG1 expression level was up-regulated in glioma cells following exposure to X-ray irradiation. FOXG1 can attenuate radiosensitivity of glioma cells. Moreover, it revealed that FOXG1 attenuate radiosensitivity of glioma cells by promoting autophagy. The present study suggests that FOXG1 is a pivotal molecule for circumventing radiation-induced cel...Continue Reading

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Citations

Feb 3, 2021·International Journal of Molecular Sciences·Imran KhanMustafa Aziz Hatiboglu
Nov 16, 2021·Frontiers in Genetics·Jinman ZhuangFei He

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