Foxj3 transcriptionally activates Mef2c and regulates adult skeletal muscle fiber type identity.

Developmental Biology
Matthew S AlexanderDaniel J Garry

Abstract

The mechanisms that regulate skeletal muscle differentiation, fiber type diversity and muscle regeneration are incompletely defined. Forkhead transcription factors are critical regulators of cellular fate determination, proliferation, and differentiation. We identified a forkhead/winged helix transcription factor, Foxj3, which was expressed in embryonic and adult skeletal muscle. To define the functional role of Foxj3, we examined Foxj3 mutant mice. Foxj3 mutant mice are viable but have significantly fewer Type I slow-twitch myofibers and have impaired skeletal muscle contractile function compared to their wild type controls. In response to a severe injury, Foxj3 mutant mice have impaired muscle regeneration. Foxj3 mutant myogenic progenitor cells have perturbed cell cycle kinetics and decreased expression of Mef2c. Examination of the skeletal muscle 5' upstream enhancer of the Mef2c gene revealed an evolutionary conserved forkhead binding site (FBS). Transcriptional assays in C2C12 myoblasts revealed that Foxj3 transcriptionally activates the Mef2c gene in a dose dependent fashion and binds to the conserved FBS. Together, these studies support the hypothesis that Foxj3 is an important regulator of myofiber identity and muscle ...Continue Reading

References

Aug 15, 1997·Developmental Biology·D J GarryR S Williams
May 3, 2000·Proceedings of the National Academy of Sciences of the United States of America·D J GarryR S Williams
Sep 1, 2000·Journal of Molecular Biology·C Pérez-SánchezJ Rey-Campos
Feb 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Zhi-Ping Liu, Eric N Olson
Sep 13, 2002·American Journal of Physiology. Cell Physiology·Robert W GrangeJames T Stull
Nov 26, 2002·The Journal of Biological Chemistry·Thomas J HawkeDaniel J Garry
Jan 10, 2003·Nucleic Acids Research·Doug StrykeThomas E Ferrin
Jun 12, 2003·Physiological Genomics·Sean C GoetschDaniel J Garry
Jun 13, 2003·Trends in Genetics : TIG·Ordan J LehmannShomi S Bhattacharya
Jun 27, 2003·American Journal of Physiology. Cell Physiology·T J HawkeD J Garry
Sep 15, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Henrik Landgren, Peter Carlsson
Feb 12, 2005·Genes & Development·Alexandre BlaisBrian David Dynlacht
Jun 1, 2005·Proceedings of the National Academy of Sciences of the United States of America·Shurong ChangEric N Olson
Aug 16, 2005·Seminars in Cell & Developmental Biology·Charlotte A Berkes, Stephen J Tapscott
Oct 21, 2005·Molecular Biology of the Cell·Timothy A McKinseyEric N Olson
Jun 24, 2006·The Biochemical Journal·Patrick J E C WijchersMarten P Smidt
Jul 5, 2006·Genes & Development·Xiaozhong Shi, Daniel J Garry
Mar 17, 2007·The EMBO Journal·Annette P MeesonDaniel J Garry
Jun 1, 2007·Development·Yaniv Hinits, Simon M Hughes
Sep 6, 2007·The Journal of Clinical Investigation·Matthew J PotthoffEric N Olson

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Citations

Dec 24, 2013·Differentiation; Research in Biological Diversity·Kathy M BowlinXiaozhong Shi
Jan 9, 2015·International Journal of Molecular Sciences·Sebastian GehlertFrank Suhr
Oct 21, 2015·Genes, Chromosomes & Cancer·Charles WaltherFredrik Mertens
Mar 24, 2010·The Biochemical Journal·Xiaozhong Shi, Daniel J Garry
Jun 22, 2012·The Biochemical Journal·Xiaozhong ShiDaniel J Garry
Dec 3, 2013·Biochimica Et Biophysica Acta·Aaron P RussellGlenn D Wadley
Feb 6, 2016·PloS One·Linyuan ShenLi Zhu
Jun 4, 2014·Molecular and Cellular Biology·Amaresh C PandaMyriam Gorospe
May 21, 2019·Oncotarget·Kiran MadugulaPooja Jain
May 24, 2019·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Qi Li, Zhengqiang Luo
Oct 11, 2012·American Journal of Physiology. Endocrinology and Metabolism·Hirotaka YamamotoHiroshi Maegawa
May 23, 2019·Proceedings of the National Academy of Sciences of the United States of America·Dounia AbbadiRobert J Schneider

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