FOXM1 and STAT3 interaction confers radioresistance in glioblastoma cells

Oncotarget
Uday B MaachaniAnita Tandle

Abstract

Glioblastoma multiforme (GBM) continues to be the most frequently diagnosed and lethal primary brain tumor. Adjuvant chemo-radiotherapy remains the standard of care following surgical resection. In this study, using reverse phase protein arrays (RPPAs), we assessed the biological effects of radiation on signaling pathways to identify potential radiosensitizing molecular targets. We identified subsets of proteins with clearly concordant/discordant behavior between irradiated and non-irradiated GBM cells in vitro and in vivo. Moreover, we observed high expression of Forkhead box protein M1 (FOXM1) in irradiated GBM cells both in vitro and in vivo. Recent evidence of FOXM1 as a master regulator of metastasis and its important role in maintaining neural, progenitor, and GBM stem cells, intrigued us to validate it as a radiosensitizing target. Here we show that FOXM1 inhibition radiosensitizes GBM cells by abrogating genes associated with cell cycle progression and DNA repair, suggesting its role in cellular response to radiation. Further, we demonstrate that radiation induced stimulation of FOXM1 expression is dependent on STAT3 activation. Co-immunoprecipitation and co-localization assays revealed physical interaction of FOXM1 wit...Continue Reading

References

May 1, 1997·Nucleic Acids Research·W KorverH Clevers
Aug 24, 1999·Cell·J F BrombergJ E Darnell
Oct 29, 1999·Genes & Development·A J PierceM Jasin
Apr 17, 2003·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·A GieseM Westphal
Feb 18, 2004·Nature Reviews. Cancer·Hua Yu, Richard Jove
Nov 19, 2004·Nature·Sheila K SinghPeter B Dirks
Jan 18, 2005·Nature Cell Biology·Jamila LaoukiliRené H Medema
Oct 18, 2005·Cancer Cell·Michael D TaylorRichard J Gilbertson
Oct 5, 2006·Cancer Research·Senthil K RadhakrishnanAndrei L Gartel
Nov 15, 2006·Molecular and Cellular Biology·Yongjun TanRobert H Costa
Dec 23, 2006·Nature Reviews. Immunology·Hua YuDrew Pardoll
Jan 16, 2007·Cancer Cell·Christopher CalabreseRichard J Gilbertson
Nov 8, 2008·Nucleic Acids Research·Mark D McDowallGeoffrey J Barton
Dec 23, 2008·International Journal of Cancer. Journal International Du Cancer·Vinita Singh-GuptaGilda G Hillman
Apr 18, 2009·Molecular Cancer Research : MCR·Amy M McCordPhilip J Tofilon
May 5, 2009·Cell Cycle·Marianna Halasi, Andrei L Gartel
Aug 13, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Amy M McCordPhilip J Tofilon
Jan 16, 2010·Expert Opinion on Investigational Drugs·Andrei L Gartel
Nov 11, 2010·Future Oncology·Walter N HittelmanLuka Milas
May 12, 2011·Neuro-oncology·Ichiro NakanoHarley I Kornblum
Jun 30, 2011·Cancer Research·Pradip Raychaudhuri, Hyun Jung Park
Oct 8, 2011·Biochimica Et Biophysica Acta·Chuay-Yeng KooEric W-F Lam
Sep 15, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Nu ZhangSuyun Huang
Mar 8, 2013·Frontiers in Oncology·Mónica Alvarez-Fernández, René H Medema
Feb 28, 2015·Molecular Cancer Research : MCR·Uday Bhanu MaachaniAnita Tandle

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Citations

Aug 9, 2017·Journal of Experimental & Clinical Cancer Research : CR·Xue ZhangHong-Hao Zhou
Feb 19, 2020·Expert Opinion on Therapeutic Targets·Soheila Borhani, Andrei L Gartel
Jul 3, 2020·Biomarkers in Medicine·Xueli LiLiyun Jia
Nov 9, 2018·Oncology Letters·Vivienne S LeeTimothy L Siu
Apr 5, 2017·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Tamara J Abou-AntounStephen M Dombrowski
May 14, 2017·Molecular Oncology·Thatchawan ThanasupawatSabine Hombach-Klonisch
Jun 27, 2019·Cancers·Carmelo GurnariEmiliano Fabiani
Apr 30, 2020·Molecular Biology of the Cell·Markaisa BlackTanya V Kalin
Sep 10, 2020·Cancers·Md Yousuf AliCorinne E Griguer
Jun 14, 2018·Experimental and Therapeutic Medicine·Xuena CuiYuanhua Ye
Jun 7, 2017·Cancer Research·Andrei L Gartel
Jan 28, 2021·Cancers·Alexander OuAmy B Heimberger
Oct 27, 2020·Cancer Biomarkers : Section a of Disease Markers·Chenglin LiYanyu Wu
Mar 9, 2021·Frontiers in Oncology·Dhanya KalathilAsha S Nair
Apr 24, 2021·ELife·Carter J BargerAdam R Karpf
Mar 11, 2021·Cancer Chemotherapy and Pharmacology·Narges K GortanyMahmoud Ghazi-Khansari
Mar 8, 2020·Cellular and Molecular Gastroenterology and Hepatology·Paula S Montenegro-MirandaVanesa Muncan
Jun 19, 2021·Frontiers in Oncology·Chiara GaleazAlessandra Bisio
Aug 7, 2021·Biochemical Pharmacology·Yun-Suk KwonSoyoung Kim

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Methods Mentioned

BETA
surgical resection
transfection
flow cytometry
immunoprecipitation

Software Mentioned

KM
Image
GeneMANIA
- Plotter
Km plotter

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