Foxm1 controls a pro-stemness microRNA network in neural stem cells

Scientific Reports
Zein Mersini BesharatElisabetta Ferretti

Abstract

Cerebellar neural stem cells (NSCs) require Hedgehog-Gli (Hh-Gli) signalling for their maintenance and Nanog expression for their self-renewal. To identify novel molecular features of this regulatory pathway, we used next-generation sequencing technology to profile mRNA and microRNA expression in cerebellar NSCs, before and after induced differentiation (Diff-NSCs). Genes with higher transcript levels in NSCs (vs. Diff-NSCs) included Foxm1, which proved to be directly regulated by Gli and Nanog. Foxm1 in turn regulated several microRNAs that were overexpressed in NSCs: miR-130b, miR-301a, and members of the miR-15~16 and miR-17~92 clusters and whose knockdown significantly impaired the neurosphere formation ability. Our results reveal a novel Hh-Gli-Nanog-driven Foxm1-microRNA network that controls the self-renewal capacity of NSCs.

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Citations

Jan 23, 2020·International Journal of Molecular Sciences·Robert Vignali, Silvia Marracci
Aug 12, 2018·International Journal of Molecular Sciences·Agnese PoZein Mersini Besharat
Apr 21, 2020·Journal of Oncology·Xiaocheng CaoJianguo Cao
Sep 14, 2018·Cell Communication and Signaling : CCS·Guo-Bin LiaoJian-Ying Bai
Oct 23, 2020·Pharmacology & Therapeutics·Martina ChiacchiariniElisabetta Ferretti

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Datasets Mentioned

BETA
MAB1637

Methods Mentioned

BETA
PCR
PCR-ChIP
ChIP
acetylation
RNA-seq
Transfection
immunoprecipitation
transfections
transfect

Software Mentioned

Primer
StatView
SDS
AxioVision Digital
- Blast
Primers Genome Analyzer
DAVID platform ( Database for Annotation , Visualization and I...
miRanda

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